Ionic liquids (ILs) are a class of substances completely comprised of ions. Due to their unique properties (e.g., electric conductivity, low volatility, thermostability and tenability), their use has attracted considerable interest, including an increasing number of publications on their use in preparing stationary phases (SPs) of high-performance liquid chromatography. We highlight the existing pathways for making IL-based SPs, and we propose potential strategies for synthesis. We also give up-to-date information on their applications in different chromatographic modes.