Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation
文献类型：会议论文
| 作者 | Li N(李宁) ; Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
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| 出版日期 | 2013 |
| 会议名称 | World Congress on Medical Physics and Biomedical Engineering |
| 会议日期 | MAY 26-31, 2012 |
| 会议地点 | Beijing, China |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | LFA-1 Mac-1 ICAM-1 on-rate off-rate |
| 卷号 | 39 |
| 页码 | 139-142 |
| 通讯作者 | 龙勉 |
| 中文摘要 | LFA-1 and Mac-1, two β2 integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type (WT) to high affinity (HA) is off-rate dependent for LFA-1 but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow. |
| 收录类别 | EI |
| 产权排序 | [Li,Ning;Mao,Debin;Gong,Yixin;Lü, Shouqin;Zhang,Yan;Long,Mian] Key Laboratory of Microgravity (National Microgravity Laboratory), Institute of Mechanics, Chinese Academy of Sciences;[Li,Ning;Mao,Debin;Gong,Yixin;Lü, Shouqin;Zhang,Yan;Long,Mian] Center of Biomechanics and Bioengineering, Institute of Mechanics, China |
| 会议网址 | http://link.springer.com/chapter/10.1007/978-3-642-29305-4_39 |
| 会议录 | World Congress on Medical Physics and Biomedical Engineering
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| 会议录出版者 | Springer Verlag,Heidelberg |
| 语种 | 英语 |
| ISSN号 | 1680-0737 |
| ISBN号 | 978-3-642-29304-7 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/48306]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Li N,Mao DB,Gong YX,et al. Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation[C]. 见:World Congress on Medical Physics and Biomedical Engineering. Beijing, China. MAY 26-31, 2012.http://link.springer.com/chapter/10.1007/978-3-642-29305-4_39. |
入库方式： OAI收割
来源：力学研究所
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