An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),
文献类型：期刊论文
| 作者 | Jinzhong Lin1 ; Jing Lu ; Yingang Feng ; Mengyi Sun ; Keqiong Ye |
| 刊名 | PLoS biology
 |
| 出版日期 | 2013 |
| 卷号 | 11期号:10页码:e1007669 |
| 中文摘要 | A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain, are components of the 90S preribosome or the small subunit processome that conducts early processing of 18S rRNA. Here, we determine the cocrystal structure of Utp22 and Rrp7 complex at 1.97 A° resolution and the NMR structure of a C-terminal fragment of Rrp7, which is not visible in the crystal structure. The structure reveals that Utp22 surprisingly resembles a dimeric class I tRNA CCA-adding enzyme yet with degenerate active sites, raising an interesting evolutionary connection between tRNA and rRNA processing machineries. Rrp7 binds extensively to Utp22 using a deviant RNA recognition motif and an extended linker. Functional sites on the two proteins were identified by structure-based mutagenesis in yeast. We show that Rrp7 contains a flexible RNA-binding C-terminal tail that is essential for association with preribosomes. RNA–protein crosslinking shows that Rrp7 binds at the central domain of 18S rRNA and shares a neighborhood with two processing H/ACA snoRNAs snR30 and snR10. Depletion of snR30 prevents the stable assembly of Rrp7 into preribosomes. Our results provide insight into the evolutionary origin and functional context of Utp22 and Rrp7. |
| 学科主题 | 代谢物组学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2014-03-20 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1564]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 推荐引用方式 GB/T 7714 | Jinzhong Lin1,Jing Lu,Yingang Feng,et al. An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),[J]. PLoS biology,2013,11(10):e1007669. |
| APA | Jinzhong Lin1,Jing Lu,Yingang Feng,Mengyi Sun,&Keqiong Ye.(2013).An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),.PLoS biology,11(10),e1007669. |
| MLA | Jinzhong Lin1,et al."An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),".PLoS biology 11.10(2013):e1007669. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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