Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.
文献类型：期刊论文
| 作者 | Li, T. ; Du, Y. ; Cui, Q. ; Zhang, J. ; Zhu, W. ; Hong, K. ; & Li, W. |
| 刊名 | Mar Drugs
 |
| 出版日期 | 2013 |
| 卷号 | 11期号:2页码:466-488 |
| 关键词 | indolocarbazole (ICZ) biosynthesis heterologous expression |
| 中文摘要 | The indolocarbazole (ICZ) alkaloids have attracted much attention due to their unique structures and potential therapeutic applications. A series of ICZs were recently isolated and identified from a marine-derived actinomycete strain, Streptomyces sanyensis FMA. To elucidate the biosynthetic machinery associated with ICZs production in S. sanyensis FMA, PCR using degenerate primers was carried out to clone the FAD-dependent monooxygenase gene fragment for ICZ ring formation, which was used as a probe to isolate the 34.6-kb DNA region containing the spc gene cluster. Sequence analysis revealed genes for ICZ ring formation (spcO, D, P, C), sugar unit formation (spcA, B, E, K, J, I), glycosylation (spcN, G), methylation (spcMA, MB), as well as regulation (spcR). Their involvement in ICZ biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152. This work represents the first cloning and characterization of an ICZ gene cluster isolated from a marine-derived actinomycete strain and would be helpful for thoroughly understanding the biosynthetic mechanism of ICZ glycosides. |
| 学科主题 | 代谢物组学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2014-03-24 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1677]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 推荐引用方式 GB/T 7714 | Li, T.,Du, Y.,Cui, Q.,et al. Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.[J]. Mar Drugs,2013,11(2):466-488. |
| APA | Li, T..,Du, Y..,Cui, Q..,Zhang, J..,Zhu, W..,...&& Li, W..(2013).Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA..Mar Drugs,11(2),466-488. |
| MLA | Li, T.,et al."Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.".Mar Drugs 11.2(2013):466-488. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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