Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method
文献类型：期刊论文
| 作者 | Song, Xiangfei1,2; Wang, Yefei1,2; Shu, Zhiyu1,2; Hong, Jingbo1,2; Li, Tong1,2; Yao, Lishan1,2 |
| 刊名 | PLOS COMPUTATIONAL BIOLOGY
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 9期号:7 |
| 中文摘要 | The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the Tm of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability. |
| 英文摘要 | The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the T-m of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 仿真与模拟 |
| 类目[WOS] | Biochemical Research Methods ; Mathematical & Computational Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Mathematical & Computational Biology |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; FREE-ENERGY CALCULATIONS ; CHARGE-CHARGE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR-DYNAMICS ; REPORTER PROTEINS ; T4 LYSOZYME ; STABILITY ; STABILIZATION ; SIMULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322320200017 |
| 公开日期 | 2014-03-21 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1634]  |
| 专题 | 青岛生物能源与过程研究所_仿真模拟团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,et al. Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method[J]. PLOS COMPUTATIONAL BIOLOGY,2013,9(7). |
| APA | Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,Hong, Jingbo,Li, Tong,&Yao, Lishan.(2013).Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method.PLOS COMPUTATIONAL BIOLOGY,9(7). |
| MLA | Song, Xiangfei,et al."Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method".PLOS COMPUTATIONAL BIOLOGY 9.7(2013). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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