An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't]
文献类型：期刊论文
| 作者 | Yu Ying ; Dongdong Meng ; Xiaohua Chen ; Fuli Li |
| 刊名 | Enzyme Microb Technol
 |
| 出版日期 | 2013 |
| 卷号 | 53期号:3页码:194-199 |
| 关键词 | Lignocellulosic biomass Caldicellulosiruptor sp. Xylanase Hyperthermophile Anaerobe |
| 中文摘要 | An anaerobic, extremely thermophilic, and cellulose- and xylan-degrading bacterium F32 was isolated from biocompost. Sequence analysis of the 16S rRNA gene of this strain showed that it was closely related to Caldicellulosiruptor saccharolyticus DSM 8903 (99.0% identity). Physiological and biochemical data also supported that identification of strain F32 as a Caldicellulosiruptor species. The proteins secreted by Caldicellulosiruptor sp. F32 grown on xylan showed a xylanase activity of 7.74 U/mg, which was 2.5 times higher than that of C. saccharolyticus DSM 8903. Based on the genomic sequencing data, 2 xylanase genes, JX030400 and JX030401, were identified in Caldicellulosiruptor sp. F32. The xylanase encoded by JX030401 shared 97% identity with Csac 0696 of C. saccharolyticus DSM 8903, while that encoded by JX030400 shared 94% identity with Athe 0089 of C. bescii DSM 6725, which was not found in the genome of strain DSM 8903. Xylanse encoded by JX030400 had 9-fold higher specific activity than JX030401. Our results indicated that although the 2 strains shared high identity, the xylanase system in Caldicellulosiruptor sp. F32 was more efficient than that in C. saccharolyticus DSM 8903. |
| 学科主题 | 微生物资源 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2014-03-20 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1559]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 推荐引用方式 GB/T 7714 | Yu Ying,Dongdong Meng,Xiaohua Chen,et al. An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't][J]. Enzyme Microb Technol,2013,53(3):194-199. |
| APA | Yu Ying,Dongdong Meng,Xiaohua Chen,&Fuli Li.(2013).An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't].Enzyme Microb Technol,53(3),194-199. |
| MLA | Yu Ying,et al."An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't]".Enzyme Microb Technol 53.3(2013):194-199. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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