Exploring the photosynthetic production capacity of sucrose by cyanobacteria
文献类型：期刊论文
| 作者 | Du, Wei1,2; Liang, Feiyan1,2 ; Duan, Yangkai1,2; Tan, Xiaoming1; Lu, Xuefeng1
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| 刊名 | METABOLIC ENGINEERING
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| 出版日期 | 2013-09-01 |
| 卷号 | 19期号:2013页码:17-25 |
| 关键词 | Sucrose Glucosylglycerol Ion-exchange chromatography Synechocystis sp PCC6803 Synechococcus elongatus PCC7942 Anabaena sp PCC7120 |
| 英文摘要 | Because cyanobacteria are photosynthetic, fast-growing microorganisms that can accumulate sucrose under salt stress, they have a potential application as a sugar source for the biomass-derived production of renewable fuels and chemicals. In the present study, the production of sucrose by the cyanobacteria Synechocystis sp. PCC6803, Synechococcus elongatus PCC7942, and Anabaena sp. PCC7120 was examined. The three species displayed different growth curves and intracellular sucrose accumulation rates in response to NaCl. Synechocystis sp. PCC6803 was used to examine the impact of modifying the metabolic pathway on the levels of sucrose production. The co-overexpression of sps (slr0045), spp (slr0953), and ugp (slr0207) lead to a 2-fold increase in intracellular sucrose accumulation, whereas knockout of ggpS (sll1566) resulted in a 1.5-fold increase in the production of this sugar When combined, these genetic modifications resulted in a fourfold increase in intracellular sucrose accumulation. To explore methods for optimizing the transport of the intracellular sucrose to the growth medium, the acid-wash technique and the CscB (sucrose permease)-dependent export method were evaluated using Synechocystis sp. PCC6803. Whereas the acid-wash technique proved to be effective, the CscB-dependent export method was not effective. Taken together, these results suggest that using genetic engineering, photosynthetic cyanobacteria can be optimized for efficient sucrose production. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 生物代谢工程 |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SYNECHOCYSTIS-SP PCC-6803 ; ESCHERICHIA-COLI ; COMPATIBLE SOLUTE ; SALT ACCLIMATION ; ACCUMULATION ; GLUCOSYLGLYCEROL ; OSMOLYTE ; STRESS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324162100003 |
| 公开日期 | 2014-03-21 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1622]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Wei,Liang, Feiyan,Duan, Yangkai,et al. Exploring the photosynthetic production capacity of sucrose by cyanobacteria[J]. METABOLIC ENGINEERING,2013,19(2013):17-25. |
| APA | Du, Wei,Liang, Feiyan,Duan, Yangkai,Tan, Xiaoming,&Lu, Xuefeng.(2013).Exploring the photosynthetic production capacity of sucrose by cyanobacteria.METABOLIC ENGINEERING,19(2013),17-25. |
| MLA | Du, Wei,et al."Exploring the photosynthetic production capacity of sucrose by cyanobacteria".METABOLIC ENGINEERING 19.2013(2013):17-25. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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