Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414
文献类型：期刊论文
| 作者 | Qi, Huan1,2,3; Bai, Fali1,2 ; Liu, Aihua1,2,3
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| 刊名 | BIOCHEMISTRY-MOSCOW
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| 出版日期 | 2013-04-01 |
| 卷号 | 78期号:4页码:424-430 |
| 关键词 | xyloglucanase cellulolytic enzymes purification Trichoderma reesei QM9414 |
| 英文摘要 | By combining anion-exchange chromatography with gel filtration, an effective method for purification of wild-type xyloglucanase and five other cellulolytic enzymes from strain QM9414 of Trichoderma reesei was established. Characterization by enzyme activity assay, SDS-PAGE, and mass spectrometry identified the purified proteins as cellobiohydrolases I and II, endoglucanases I and II, a xyloglucanase, and beta-xylosidase, of which the xyloglucanase was purified for the first time from the mutant strain QM9414. This method holds great promise to study the mechanism of cellulolytic enzymes, to investigate the synergistic action between cellulase and other cellulolytic enzymes, and to better exploit enzyme preparations for degradation of lignocellulose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 生物传感器技术 |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | BETA-GLUCOSIDASE ; THERMOSTABLE XYLANASES ; DEGRADING ENZYMES ; CELLULASE ; ENDOGLUCANASES ; XYLOSIDASE ; HYDROLYSIS ; CHROMATOGRAPHY ; FRACTIONATION ; QM-9414 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317645000012 |
| 公开日期 | 2014-03-24 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1690]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Huan,Bai, Fali,Liu, Aihua. Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414[J]. BIOCHEMISTRY-MOSCOW,2013,78(4):424-430. |
| APA | Qi, Huan,Bai, Fali,&Liu, Aihua.(2013).Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414.BIOCHEMISTRY-MOSCOW,78(4),424-430. |
| MLA | Qi, Huan,et al."Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414".BIOCHEMISTRY-MOSCOW 78.4(2013):424-430. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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