Evaluation of DNA Extraction and Purification Methods for Corn Straw Biogas Slurry
文献类型：期刊论文
| 作者 | Qiao, J. T ; Guo, R. B ; Shi, X. S ; Qiu, Y. L. |
| 刊名 | Advanced Materials Research
 |
| 出版日期 | 2013 |
| 期号 | 773页码:113-117 |
| 关键词 | Corn straw biogas slurry Molecular ecology DNA extraction PCR RFLP |
| 中文摘要 | Corn straw biogas slurry always contains humic substances, which poses particular challenges in obtaining PCR-amplifiable DNA for analysis of microbial community. To establish an efficient and reliable DNA extraction method for straw biogas slurry, four approaches: i.e., direct SDS-based method, direct bead-based method, indirect SDS-based method, and indirect bead-based method were evaluated by comparing DNA yield, humic acid contamination, PCR amplifiability, and restriction fragment length polymorphisms (RFLP) of amplified 16S rRNA genes. Direct DNA extraction methods yielded 3-fold higher amounts of DNA than indirect procedures, but its DNA purity was lower. The A260/A230 ratio of DNA from indirect methods (0.8-0.85) were higher than that of DNA from direct methods (0.5-0.6), indicating DNA from direct methods contained high levels of humate contamination. PCR amplification was successful with crude DNA from indirect methods, but not with crude DNA from direct methods. PCR products could also be obtained with purified DNA from direct bead-based method, whereas not direct SDS-based method. Among the four methods, direct bead-based method, indirect SDS-based method and indirect bead-based method could obtain high-quality DNA extracts from corn straw biogas slurry. RFLP analysis further demonstrated the restriction patterns of amplified 16S rRNA genes from three methods were relatively identical microbial diversity. |
| 学科主题 | 生物制氢与沼气 |
| 收录类别 | EI |
| 语种 | 英语 |
| 公开日期 | 2014-03-21 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1612]  |
| 专题 | 青岛生物能源与过程研究所_生物制氢与沼气团队 |
| 推荐引用方式 GB/T 7714 | Qiao, J. T,Guo, R. B,Shi, X. S,et al. Evaluation of DNA Extraction and Purification Methods for Corn Straw Biogas Slurry[J]. Advanced Materials Research,2013(773):113-117. |
| APA | Qiao, J. T,Guo, R. B,Shi, X. S,&Qiu, Y. L..(2013).Evaluation of DNA Extraction and Purification Methods for Corn Straw Biogas Slurry.Advanced Materials Research(773),113-117. |
| MLA | Qiao, J. T,et al."Evaluation of DNA Extraction and Purification Methods for Corn Straw Biogas Slurry".Advanced Materials Research .773(2013):113-117. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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