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Induction of gene expression in bacteria at optimal growth temperatures

文献类型：期刊论文

作者Jiang, Xinglin1,2; Zhang, Haibo1; Yang, Jianming1; Liu, Min3; Feng, Hongru4; Liu, Xiaobin5; Cao, Yujin1; Feng, Dexin1; Xian, Mo1
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2013-06-01
卷号97期号:12页码:5423-5431
关键词Expression system Temperature induction cI857 lacI Acetoin
英文摘要Traditional temperature-sensitive systems use either heat shock (40-42 A degrees C) or cold shock (15-23 A degrees C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 A degrees C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we have developed a new system which controls gene expression in Escherichia coli at more permissive temperatures. The temperature-sensitive cI857-P (L) system and the classic lacI-P (lacO) system were connected in series to control the gene of interest. When the culture temperature was lowered, the thermolabile cI857 repressor was activated and blocked the expression of lacI from P (L). Subsequently, the decrease of LacI derepressed the expression of gene of interest from P (lacO) . Using a green fluorescent protein marker, we demonstrated that (1) gene expression was tightly regulated at 42 A degrees C and strongly induced by lowering temperature to 25-37 A degrees C; (2) different levels of gene expression can be induced by varying culture temperature; and (3) gene expression after induction was sustained until the end of the log phase. We then applied this system in the biosynthesis of acetoin and demonstrated that high yield and production could be achieved using temperature induction. The ability to express proteins at optimal growth temperatures without chemical inducers is advantageous for large-scale and industrial fermentations.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题生物基化学品
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]HETEROLOGOUS PROTEIN-PRODUCTION ; ESCHERICHIA-COLI ; CLONING VECTORS ; SYSTEM ; BIOSYNTHESIS ; BIOFUELS ; ACETOIN ; SINGLE ; CSPA
收录类别SCI
语种英语
WOS记录号WOS:000319609400022
公开日期2014-03-24
源URL[http://ir.qibebt.ac.cn:8080/handle/337004/1665]  
专题青岛生物能源与过程研究所_材料生物技术研究中心
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Wuhan Univ Sci & Technol, Coll Chem Engn & Technol, Wuhan 430081, Peoples R China
4.Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110866, Peoples R China
5.Jiangxi Agr Univ, Coll Sci, Nanchang 330045, Jiangxi, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Xinglin,Zhang, Haibo,Yang, Jianming,et al. Induction of gene expression in bacteria at optimal growth temperatures[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(12):5423-5431.
APA Jiang, Xinglin.,Zhang, Haibo.,Yang, Jianming.,Liu, Min.,Feng, Hongru.,...&Xian, Mo.(2013).Induction of gene expression in bacteria at optimal growth temperatures.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(12),5423-5431.
MLA Jiang, Xinglin,et al."Induction of gene expression in bacteria at optimal growth temperatures".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.12(2013):5423-5431.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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