Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli
文献类型：期刊论文
| 作者 | Wang, Qi1,2; Yang, Peng1,3; Liu, Changshui1,2; Xue, Yongchang3; Xian, Mo1 ; Zhao, Guang1
|
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 131期号:2013页码:548-551 |
| 关键词 | Poly(3-hydroxypropionate) Glycerol dehydratase Propionaldehyde dehydrogenase Glycerol-glucose cofermentation |
| 英文摘要 | Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. After culture condition optimization, the final engineered strain accumulated 10.1 g/L P3HP (46.4% of the cell dry weight) using glycerol and glucose as cosubstrates in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without addition of any expensive precursor. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 学科主题 | 生物基化学品 |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | BACTERIAL POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYPROPIONATE) ; 3-HYDROXYPROPIONIC ACID ; REACTIVATING FACTOR ; DEHYDRATASE ; POLYESTERS ; MECHANISM ; PATHWAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317024200074 |
| 公开日期 | 2014-03-24 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1669]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Yang, Peng,Liu, Changshui,et al. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2013,131(2013):548-551. |
| APA | Wang, Qi,Yang, Peng,Liu, Changshui,Xue, Yongchang,Xian, Mo,&Zhao, Guang.(2013).Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli.BIORESOURCE TECHNOLOGY,131(2013),548-551. |
| MLA | Wang, Qi,et al."Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli".BIORESOURCE TECHNOLOGY 131.2013(2013):548-551. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。