Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli
文献类型：期刊论文
| 作者 | Zou, Huibin; Wu, Zaiqiang; Xian, Mo ; Liu, Hui; Cheng, Tao; Cao, Yujin
|
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 148期号:148c页码:591-595 |
| 关键词 | L-Lactic acid Lactobacilli Betaine Osmoprotectant Lactate dehydrogenase activity |
| 英文摘要 | Lactobacilli are commonly used for industrial production of polymer-grade L-lactic acid. The present study tested the Tween 80 alternative betaine in L-lactate production by several industrial lactobacilli. In flask fermentation of Lactobacillus casei, Lactobacillus buchneri, Lactobacillus lactis and Lactobacillus rhamnosus, the betaine addition (2 g/l) had similar osmoprotectant effect with Tween 80 but had increased the lactate dehydrogenase activities and L-lactate production than Tween 80 control. In fed-batch fermentation of L. casei, betaine supplementation improved the L-lactic acid titer to 190 g/l, the yield to 95.5% (g L-lactic acid/g glucose), the productivity to 2.6 g/l h, and the optical purity to 97.0%. The results demonstrated that supplementation of Tween 80 alternative - betaine in the fermentation medium is feasible for industrial L-lactic acid fermentation by lactobacilli, which will improve the lactate production but will not increase the process costs and modify any process conditions. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 学科主题 | 生物基化学品 |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | L-LACTIC ACID ; GLYCINE BETAINE ; EFFICIENT PRODUCTION ; BACILLUS-SUBTILIS ; GROWTH ; FERMENTATION ; MODULATION ; TOLERANCE ; SURVIVAL ; PURITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326905400080 |
| 公开日期 | 2014-03-24 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1678]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Huibin,Wu, Zaiqiang,Xian, Mo,et al. Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli[J]. BIORESOURCE TECHNOLOGY,2013,148(148c):591-595. |
| APA | Zou, Huibin,Wu, Zaiqiang,Xian, Mo,Liu, Hui,Cheng, Tao,&Cao, Yujin.(2013).Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli.BIORESOURCE TECHNOLOGY,148(148c),591-595. |
| MLA | Zou, Huibin,et al."Not only osmoprotectant: Betaine increased lactate dehydrogenase activity and L-lactate production in lactobacilli".BIORESOURCE TECHNOLOGY 148.148c(2013):591-595. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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