Identification and Molecular Characterization of FKF1 and GI Homologous Genes in Soybean
文献类型：期刊论文
| 作者 | Li, Fang1; Zhang, Xiaomei1; Hu, Ruibo2 ; Wu, Faqiang1; Ma, Jinhua1; Meng, Ying1; Fu, YongFu1
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-11-13 |
| 卷号 | 8期号:11 |
| 中文摘要 | In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1a and GmGI1b. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants. |
| 英文摘要 | In Arabidopsis, FKF1 (FLAVIN BINDING, KELCH REPEAT, F-BOX1) and GI (GIGANTEA) play important roles in flowering pathway through regulating daytime CO (CONSTANS) expression, and such a function is conserved across plants studied. But related reports are limited for soybean. In this study, we cloned FKF1 and GI homologs in soybean, and named as GmFKF1, GmFKF2, GmGI1, GmGI2, and GmGI3, respectively. GmGI1 had two alternative splicing forms, GmGI1 alpha and GmGI1 beta. GmFKF1/2 transcripts were diurnally regulated, with a peak at zeitgeber time 12 (ZT12) in long days and at ZT10 in short days. The diurnal phases between GmGIs transcript levels greatly differed. GmGI2 expression was regulated by both the circadian clock and photoperiod. But the rhythmic phases of GmGI1 and GmGI3 expression levels were mainly conferred by long days. GmFKFs shared similar spatio-temporal expression profiles with GmGIs in all of the tissue/organs in different developmental stages in both LD and SD. Both GmFKF and GmGI proteins were targeted to the nucleus. Yeast two hybrid assays showed GmFKF1/GmFKF2 interacted with GmGI1/GmGI2/GmCDF1 (CYCLING DOF FACTOR CDF1 homolog in soybean); and the LOV (Light, Oxygen, or Voltage) domain in GmFKF1/GmFKF2 played an important role in these interactions. N-terminus of GmGI2 was sufficient to mediate its interaction with GmCDF1. Interestingly, N-terminus not full of GmGI3 interacted with GmFKF1/GmFKF2/GmCDF1. Ectopic over-expression of the GmFKF1 or GmFKF2 in Arabidopsis enhanced flowering in SD. Collectively, GmFKF and GmGI in soybean had conserved functional domains at DNA sequence level, but specific characters at function level with their homologs in other plants. |
| WOS标题词 | Science & Technology |
| 学科主题 | 微生物资源 |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | F-BOX PROTEINS ; ARABIDOPSIS CIRCADIAN CLOCK ; FLOWERING-LOCUS-T ; MAP-BASED CLONING ; GIGANTEA GENE ; CONFORMATIONAL-CHANGES ; GROWTH HABIT ; EXPRESSION ; CONSTANS ; THALIANA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327254700084 |
| 公开日期 | 2014-03-21 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1584]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resource & Genet Improve, MOA Key Lab Soybean Biol Beijing, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fang,Zhang, Xiaomei,Hu, Ruibo,et al. Identification and Molecular Characterization of FKF1 and GI Homologous Genes in Soybean[J]. PLOS ONE,2013,8(11). |
| APA | Li, Fang.,Zhang, Xiaomei.,Hu, Ruibo.,Wu, Faqiang.,Ma, Jinhua.,...&Fu, YongFu.(2013).Identification and Molecular Characterization of FKF1 and GI Homologous Genes in Soybean.PLOS ONE,8(11). |
| MLA | Li, Fang,et al."Identification and Molecular Characterization of FKF1 and GI Homologous Genes in Soybean".PLOS ONE 8.11(2013). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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