MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering
文献类型：期刊论文
| 作者 | Yu, Yanchong; Hu, Ruibo; Wang, Huamei; Cao, Yingping; He, Guo; Fu, Chunxiang; Zhou, Gongke |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 212期号:212页码:1-9 |
| 关键词 | MlWRKY12 Miscanthus Secondary cell wall Flowering time |
| 中文摘要 | WRKY proteins play crucial roles in various plant processes. An AtWRKY12 homologous gene, namedMlWRKY12, was isolated from Miscanthus lutarioriparius. The MlWRKY12 gene encodes a WRKY transcrip-tion factor belonging to the group IIc subfamily. MlWRKY12 is a nuclear protein. Gene expression patternanalysis revealed a relatively high MlWRKY12 expression level in rhizomes, stems and leaf sheaths. In situhybridization analysis further demonstrated that MlWRKY12 was expressed in vascular bundle sheath,sclerenchyma and parenchyma tissues. The heterologous expression of MlWRKY12 in an atwrky12 back-ground mutant successfully rescued the phenotype of pith cell walls caused by the defect of AtWRKY12.Most strikingly, the transgenic Arabidopsis plants overexpressing MlWRKY12 exhibited early flowering.The transcript abundance of flowering related genes was measured by quantitative RT-PCR analysis,suggesting that overexpression of MlWRKY12 in Arabidopsis had a significant impact on the expres-sion level of CONSTANS (CO). Moreover, the expression levels of FLOWERING LOCUS T (FT), LFY (LEAFY),APETALA1 (AP1), CAULIFLOWER (CAL) and FRUITFULL (FUL) were upregulated in transgenic plants. Theseresults demonstrated the conserved function of MlWRKY12 existing in secondary cell wall formation ofmonocotyledonous species and implied a possible impact of MlWRKY12 on flowering control. |
| 英文摘要 | WRKY proteins play crucial roles in various plant processes. An AtWRKY12 homologous gene, named M, was isolated from Miscanthus lutarioriparius. The MlWRKY12 gene encodes a WRKY transcription factor belonging to the group llc subfamily. MlWRKY12 is a nuclear protein. Gene expression pattern analysis revealed a relatively high MlWRKY12 expression level in rhizomes, stems and leaf sheaths. In situ hybridization analysis further demonstrated that MlWRKY12 was expressed in vascular bundle sheath, sclerenchyma and parenchyma tissues. The heterologous expression of MlWRKY12 in an atwrky12 background mutant successfully rescued the phenotype of pith cell walls caused by the defect of AtWRKY12. Most strikingly, the transgenic Arabidopsis plants overexpressing MlWRKY12 exhibited early flowering. The transcript abundance of flowering related genes was measured by quantitative RT-PCR analysis, suggesting that overexpression of MlWRKY12 in Arabidopsis had a significant impact on the expression level of CONSTANS (CO). Moreover, the expression levels of FLOWERING LOCUST (FT), LFY (LEAFY), APETALA1 (AP1), CAULIFLOWER (CAL) and FRUITFULL (FUL) were upregulated in transgenic plants. These results demonstrated the conserved function of MlWRKY12 existing in secondary cell wall formation of monocotyledonous species and implied a possible impact of MlWRKY12 on flowering control. (C) 2013 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 能源植物资源 |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | ARABIDOPSIS-THALIANA ; SINENSIS ANDERSS ; LEAF SENESCENCE ; ENERGY CROP ; SWITCHGRASS ; SIGNALS ; PLANTS ; IDENTIFICATION ; PATHWAY ; BIOMASS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326133500001 |
| 公开日期 | 2014-03-21 |
| 源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1591]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | Chinese Acad Sci QIBEBT CAS, Key Lab Biofuels, Inst BioEnergy & Bioproc Technol, Chinese Acad Sci, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Yanchong,Hu, Ruibo,Wang, Huamei,et al. MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering[J]. PLANT SCIENCE,2013,212(212):1-9. |
| APA | Yu, Yanchong.,Hu, Ruibo.,Wang, Huamei.,Cao, Yingping.,He, Guo.,...&Zhou, Gongke.(2013).MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering.PLANT SCIENCE,212(212),1-9. |
| MLA | Yu, Yanchong,et al."MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering".PLANT SCIENCE 212.212(2013):1-9. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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