中国科学院机构知识库网格系统: Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)

Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)

文献类型：期刊论文


作者	Zuo, Ran 1; Hu, Ruibo1 ; Chai, Guohua1 ; Xu, Meiling 1; Qi, Guang1 ; Kong, Yingzhen 2; Zhou, Gongke1
刊名	MOLECULAR BIOLOGY REPORTS
出版日期	2013-03-01
卷号	40 期号:3 页码:2645-2662
关键词	Populus trichocarpa CDPK Phylogenetic analysis Gene structure Expression analysis
中文摘要	Calcium-dependent protein kinases (CDPKs) areCa2?-binding proteins known to play crucial roles in Ca2?signal transduction pathways which have been identified throughout plant kingdom and in certain types of protists.Genome-wide analysis of CDPKs have been carried out in Arabidopsis, rice and wheat, and quite a few of CDPKs were proved to play crucial roles in plant stress responsive signature pathways. In this study, a comprehensive analysis of Populus CDPK and its closely related gene families was performed, including phylogeny, chromosome locations, gene structures, and expression profiles. Thirty Populus CDPK genes and twenty closely related kinase genes were identified, which were phylogenetically clustered into eight distinct subfamilies and predominately distributed across fifteen linkage groups (LG). Genomic organization analyses indicated that purifying selection has played a pivotal role in the retention andmaintenance of PopulusCDPKgene family. Furthermore, microarray analysis showed that a number of Populus CDPK and its closely related genes differentially expressed across disparate tissues and under various stresses. The expression profiles of paralogous pairs were also investigated to reveal their evolution fates. In addition, quantitative real-time RT-PCR was performed on nine selected CDPK genes to confirm their responses to drought stress treatment. These observations may lay the foundation for future functional analysis of Populus CDPK and its closely related gene families to unravel their biological roles.
英文摘要	Calcium-dependent protein kinases (CDPKs) are Ca2+-binding proteins known to play crucial roles in Ca2+ signal transduction pathways which have been identified throughout plant kingdom and in certain types of protists. Genome-wide analysis of CDPKs have been carried out in Arabidopsis, rice and wheat, and quite a few of CDPKs were proved to play crucial roles in plant stress responsive signature pathways. In this study, a comprehensive analysis of Populus CDPK and its closely related gene families was performed, including phylogeny, chromosome locations, gene structures, and expression profiles. Thirty Populus CDPK genes and twenty closely related kinase genes were identified, which were phylogenetically clustered into eight distinct subfamilies and predominately distributed across fifteen linkage groups (LG). Genomic organization analyses indicated that purifying selection has played a pivotal role in the retention and maintenance of Populus CDPK gene family. Furthermore, microarray analysis showed that a number of Populus CDPK and its closely related genes differentially expressed across disparate tissues and under various stresses. The expression profiles of paralogous pairs were also investigated to reveal their evolution fates. In addition, quantitative real-time RT-PCR was performed on nine selected CDPK genes to confirm their responses to drought stress treatment. These observations may lay the foundation for future functional analysis of Populus CDPK and its closely related gene families to unravel their biological roles.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
学科主题	能源植物资源
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	DEPENDENT-PROTEIN-KINASE ; CALMODULIN-LIKE DOMAIN ; ARABIDOPSIS-THALIANA ; CALCIUM SENSORS ; ABSCISIC-ACID ; BIOCHEMICAL-CHARACTERIZATION ; COMPREHENSIVE ANALYSIS ; MAXIMUM-LIKELIHOOD ; DUPLICATED GENES ; MICROARRAY DATA
收录类别	SCI
语种	英语
WOS记录号	WOS:000314535600064
公开日期	2014-03-21
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1593]
专题	青岛生物能源与过程研究所_植物代谢工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 2.Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
推荐引用方式 GB/T 7714	Zuo, Ran,Hu, Ruibo,Chai, Guohua,et al. Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)[J]. MOLECULAR BIOLOGY REPORTS,2013,40(3):2645-2662.
APA	Zuo, Ran.,Hu, Ruibo.,Chai, Guohua.,Xu, Meiling.,Qi, Guang.,...&Zhou, Gongke.(2013).Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa).MOLECULAR BIOLOGY REPORTS,40(3),2645-2662.
MLA	Zuo, Ran,et al."Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)".MOLECULAR BIOLOGY REPORTS 40.3(2013):2645-2662.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa)

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