For observing thousands of celestial bodies simultaneously, 4000 nodes with an alveolate distribution on the focal plate of 1.75m in diameter are used in Large Sky Area Multi-Object Fiber Spectroscopy Telescope (LAMOST). In this paper, a novel fiber positioning control method based on ZigBee PRO was proposed for LAMOST fiber positioning. Compared with traditional control way of wire drive, it can reduce the number of external antenna more than 95%, furthermore, the difficulty of installation was minimized by saving the space on the back of focal plate. ZigBee PRO networking and data communication technology are analyzed and a mesh network is designed based on LM3S9B96, CC2520 and CC2530 to realize localization and reset for 4000 fiber positioning units. The fiber positioning control computer can make the further decision by employing of the feedback information of zero point.