Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus
文献类型：期刊论文
| 作者 | Liu NN ; Chen Y ; Peng B ; Lin Y ; Wang Q ; Su ZH ; Zhang WK ; Li HB ; Shen JC |
| 刊名 | biophysical journal
 |
| 出版日期 | 2013 |
| 卷号 | 105期号:12页码:2790-2800 |
| 关键词 | RECOGNITION EVENTS ENERGY LANDSCAPES RECENT PROGRESS MICROSCOPY PROTEIN RESOLUTION ADHESION SEQUENCE BINDING CELLS |
| ISSN号 | 0006-3495 |
| 通讯作者 | wang q |
| 中文摘要 | to explore the disassembly mechanism of tobacco mosaic virus (tmv), a model system for virus study, during infection, we have used single-molecule force spectroscopy to mimic and follow the process of rna disassembly from the protein coat of tmv by the replisome (molecular motor) in vivo, under different ph and ca-2+ concentrations. dynamic force spectroscopy revealed the unbinding free-energy landscapes as that at ph 4.7 the disassembly process is dominated by one free-energy barrier, whereas at ph 7.0 the process is dominated by one barrier and that there exists a second barrier. the additional free-energy barrier at longer distance has been attributed to the hindrance of disordered loops within the inner channel of tmv, and the biological function of those protein loops was discussed. the combination of ph increase and ca2+ concentration drop could weaken rna-protein interactions so much that the molecular motor replisome would be able to pull and disassemble the rest of the genetic rna from the protein coat in vivo. all these facts provide supporting evidence at the single-molecule level, to our knowledge for the first time, for the cotranslational disassembly mechanism during tmv infection under physiological conditions. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000328597400024 |
| 公开日期 | 2014-04-14 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49388]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu NN,Chen Y,Peng B,et al. Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus[J]. biophysical journal,2013,105(12):2790-2800. |
| APA | Liu NN.,Chen Y.,Peng B.,Lin Y.,Wang Q.,...&Shen JC.(2013).Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus.biophysical journal,105(12),2790-2800. |
| MLA | Liu NN,et al."Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus".biophysical journal 105.12(2013):2790-2800. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。