Polylysine-modified polyethylenimine inducing tumor apoptosis as an efficient gene carrier
文献类型：期刊论文
| 作者 | Tian HY ; Lin L ; Jiao ZX ; Guo ZP ; Chen J ; Gao SQ ; Zhu XJ ; Chen XS |
| 刊名 | journal of controlled release
 |
| 出版日期 | 2013 |
| 卷号 | 172期号:2页码:410-418 |
| 关键词 | SIRNA CARRIER IN-VITRO DELIVERY VECTORS POLYMER CELLS VIRUS NANOPARTICLES TRANSFECTION COMPLEXES |
| ISSN号 | 0168-3659 |
| 通讯作者 | zhu xj |
| 中文摘要 | polyethylenimine (pei) is receiving increasing attention as a gene carrier with high transfection efficiency. however, its high charge density and cytotoxic effects limit its application. polylysine (pll) is another polymeric gene carrier with good biodegradability and biocompatibility, although its lack of endosomal escape ability strongly impairs its transfection efficiency. in this study, pll was introduced to pei by ring-opening polymerization of epsilon-benzyloxycarbonyl-l-lysine n-carboxyanhydride, followed by deprotection of carbobenzyloxy groups. as-prepared pei-pll multiarm hyperbranched copolymers were characterized as gene carriers in vitro by measuring their particle size, zeta potential, cytotoxicity, transfection efficiency, and cell internalization. the optimum transfected efficiency of pei-pll was nearly seven times higher than that of pei with a molecular weight of 25 kda. furthermore, pkh3-rev-casp-3 plasmid dna was used as a gene for anti-tumor treatment in a xenograft model using nude mice. compared with 25 kda pei, pei-pll exhibited better tumor inhibition effects in 23 days. in addition, terminal deoxynucleotidyl transferase dutp nick end labeling, immunohistochemistry, and western blot analysis were used to determine the anti-tumor mechanism of pei-pll. the results showed that tumor cell apoptosis led to tumor inhibition, which could be attributed to pkh3-rev-casp-3 inducing poly(adp-ribose) polymerase-1 cleavage. pei-pll is a promising gene carrier candidate for further application in vivo. (c) 2013 published by elsevier b.v. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000327601700004 |
| 公开日期 | 2014-04-14 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49403]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tian HY,Lin L,Jiao ZX,et al. Polylysine-modified polyethylenimine inducing tumor apoptosis as an efficient gene carrier[J]. journal of controlled release,2013,172(2):410-418. |
| APA | Tian HY.,Lin L.,Jiao ZX.,Guo ZP.,Chen J.,...&Chen XS.(2013).Polylysine-modified polyethylenimine inducing tumor apoptosis as an efficient gene carrier.journal of controlled release,172(2),410-418. |
| MLA | Tian HY,et al."Polylysine-modified polyethylenimine inducing tumor apoptosis as an efficient gene carrier".journal of controlled release 172.2(2013):410-418. |
入库方式： OAI收割
来源：长春应用化学研究所
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