Rapid high-throughput analysis of ochratoxin A by the self-assembly of DNAzyme-aptamer conjugates in wine
文献类型：期刊论文
| 作者 | Yang C ; Lates V ; Prieto-Simon B ; Marty JL ; Yang XR |
| 刊名 | talanta
 |
| 出版日期 | 2013 |
| 卷号 | 116页码:520-526 |
| 关键词 | WHEAT DNA |
| ISSN号 | 0039-9140 |
| 通讯作者 | yang xr |
| 中文摘要 | we report a new label-free calorimetric aptasensor based on dnazyme-aptamer conjugate for rapid and high-throughput detection of ochratoxin a (ota, a possible human carcinogen, group 2b) in wine. two oligonucleotides were designed for this detection. one is n1 for biorecognition, which includes two adjacent sequences: the ota-specific aptamer sequence and the horseradish peroxidase (hrp)-mimicking dnazyme sequence. the other is a blocking dna (b2), which is partially complementary to a part of the ota aptamer and partially complementary to a part of the dnazyme. the existence of ota reduces the hybridization between n1 and b2. thus, the activity of the non-hybridized dnazyme is linearly correlated with the concentration of ota up to 30 nm with a limit of detection of 4 nm (3 sigma). meanwhile, a double liquid-liquid extraction (lle) method is accordingly developed to purify ota from wine. compared with the existing hplc-fd or immunoassay methods, the proposed strategy presents the most appropriate balance between accuracy and facility, resulting in a considerable improvement of real-time quality control, and thereby, preventing chronic poisoning caused by ota contained red wine. (c) 2013 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000328176000076 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49463]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang C,Lates V,Prieto-Simon B,et al. Rapid high-throughput analysis of ochratoxin A by the self-assembly of DNAzyme-aptamer conjugates in wine[J]. talanta,2013,116:520-526. |
| APA | Yang C,Lates V,Prieto-Simon B,Marty JL,&Yang XR.(2013).Rapid high-throughput analysis of ochratoxin A by the self-assembly of DNAzyme-aptamer conjugates in wine.talanta,116,520-526. |
| MLA | Yang C,et al."Rapid high-throughput analysis of ochratoxin A by the self-assembly of DNAzyme-aptamer conjugates in wine".talanta 116(2013):520-526. |
入库方式： OAI收割
来源：长春应用化学研究所
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