Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing
文献类型：期刊论文
| 作者 | Wang Y ; Chen J ; Jiao HP ; Chen Y ; Li WY ; Zhang QF ; Yu C |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2013 |
| 卷号 | 19期号:38页码:12846-12852 |
| 关键词 | ALKALINE-PHOSPHATASE ACTIVITY AGGREGATION-INDUCED EMISSION CONJUGATED POLYELECTROLYTES GOLD NANOPARTICLES ASSAY DNA BIOCHEMISTRY SENSORS SILOLE WATER |
| ISSN号 | 0947-6539 |
| 通讯作者 | yu c |
| 中文摘要 | a new label-free fluorescence turn-on strategy for highly sensitive biosensing has been developed. a negatively charged perylene probe was synthesized. polycations could induce aggregation of the perylene probe through noncovalent interactions and the fluorescence of the probe's monomer was efficiently quenched. upon addition of a single-stranded nucleic acid, competitive binding of the negatively charged nucleic acid (a polyanion) to the cationic polymer resulted in the release of a monomer and thus a turn-on fluorescence signal was detected. without the use of any amplification techniques, a detection limit of 2pm dna was obtained. based on these results, an assay strategy for the highly sensitive detection of alkaline phosphatase (alp) activity has been demonstrated. exonuclease (exo) could degrade 5-phosphorylated single-stranded dna. however, when the dna sample was treated with alp, the phosphate functional group was removed by alp and it could no longer be degraded by exo. binding of the dna to the perylene probe-polycation complex resulted in a turn-on fluorescence signal, which could be used for sensing of alp. the method is highly sensitive, a limit of detection as low as 0.02muml(-1) alp was obtained. our method is simple, convenient, highly sensitive, and inexpensive. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324316300039 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49612]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang Y,Chen J,Jiao HP,et al. Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing[J]. chemistry-a european journal,2013,19(38):12846-12852. |
| APA | Wang Y.,Chen J.,Jiao HP.,Chen Y.,Li WY.,...&Yu C.(2013).Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing.chemistry-a european journal,19(38),12846-12852. |
| MLA | Wang Y,et al."Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing".chemistry-a european journal 19.38(2013):12846-12852. |
入库方式： OAI收割
来源：长春应用化学研究所
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