Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Wang CY ; Zhu HB ; Pi ZF ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2013 |
| 卷号 | 935页码:26-31 |
| 关键词 | IMPAIRED GLUCOSE-TOLERANCE INHERITED DISORDERS BIOLOGICAL INTEREST MS/MS ANALYSIS INBORN-ERRORS BODY-FLUIDS LIFE-STYLE ESI-MS/MS DIAGNOSIS TOOL |
| ISSN号 | 1570-0232 |
| 通讯作者 | song fr |
| 中文摘要 | an analytical method for quantifying underivatized amino acids (aas) in urine samples of rats was developed by using liquid chromatography coupled with tandem mass spectrometry (lc-ms/ms). classification of type 2 diabetes rats was based on urine amino acids metabolic profiling. lc-ms/ms analysis was applied through chromatographic separation and multiple reactions monitoring (mrm) transitions of ms/ms. multivariate profile-wide predictive models were constructed using partial least squares discriminant analysis (pls-da) by simac-p 11.5 version software package and hierarchical cluster analysis (hca) by spss 18.0 version software. some amino acids in urine of rats have significant change. the results of the present study prove that this method could perform the quantification of free aas in urine of rats by using lc-ms/ms. in summary, the pls-da and hca statistical analysis in our research were preferable to differentiate healthy rats and type 2 diabetes rats by the quantification of aas in their urine samples. in addition, comparing with health group the seven increased amino acids in urine of type 2 rats were returned to normal under the treatment of acarbose. (c) 2013 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324084200004 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49613]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang CY,Zhu HB,Pi ZF,et al. Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2013,935:26-31. |
| APA | Wang CY,Zhu HB,Pi ZF,Song FR,Liu ZQ,&Liu SY.(2013).Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry.journal of chromatography b-analytical technologies in the biomedical and life sciences,935,26-31. |
| MLA | Wang CY,et al."Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry".journal of chromatography b-analytical technologies in the biomedical and life sciences 935(2013):26-31. |
入库方式： OAI收割
来源：长春应用化学研究所
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