Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples
文献类型：期刊论文
| 作者 | Xu C ; Liu S ; Liu ZQ ; Song FR ; Liu SY |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2013 |
| 卷号 | 793页码:53-60 |
| ISSN号 | 0003-2670 |
| 通讯作者 | liu s |
| 中文摘要 | superoxide anion radical (o-2 radical anion) plays an important role in several human diseases. the xanthine/xanthine oxidase system is frequently utilized to produce o-2 radical anion. however, false positive results are easily got by using this system. the common spectrophotometric probes for o-2 radical anion are nitroblue tetrazolium (nbt) and cytochrome c. nevertheless, the application of nbt method is limited because of the water-insolubility of nbt formazan and the assay using cytochrome c lacks sensitivity and is not suitable for microplate measurement. we overcome these problems by using 1,2,3-trihydroxybenzene (pyrogallol) as o-2 radical anion-generating system and a highly water-soluble tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt (wst-1) which can be reduced by superoxide anion radical to a stable water-soluble formazan with a high absorbance at 450 nm. the method is simple, rapid and sensitive. moreover, it can be adapted to microplate format. in this study, the o-2 radical anion scavenging activities of superoxide dismutase (sod), l-ascorbic acid, n-acetyl-l-cysteine (nac), albumin from human serum, flavonoids and herbal extracts were assessed by using this method. meanwhile, the activities of tissue homogenates and serum were determined by using this validated method. this method, applicable to tissue homogenates, serum and herbal extracts, proved to be efficient for measuring o-2 radical anion scavenging activities of biological and abiological samples. (c) 2013 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323855400007 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49629]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xu C,Liu S,Liu ZQ,et al. Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples[J]. analytica chimica acta,2013,793:53-60. |
| APA | Xu C,Liu S,Liu ZQ,Song FR,&Liu SY.(2013).Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples.analytica chimica acta,793,53-60. |
| MLA | Xu C,et al."Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples".analytica chimica acta 793(2013):53-60. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。