Development of Light Waveguide Resonance Light-Scattering Scanner for Microarray Detection
文献类型：期刊论文
| 作者 | Xing XF ; Liu X ; Li T ; Wang ZX |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 41期号:9页码:1458-1462 |
| 关键词 | GOLD NANOPARTICLE PROBES PROTEIN-KINASE ACTIVITY ASSAY FUNCTIONALITY BINDING SURFACE |
| ISSN号 | 0253-3820 |
| 通讯作者 | wang zx |
| 中文摘要 | an optical waveguide resonance light-scattering scanner (rls scanner) was developed for microarray detection. the major components of the device included optical system, waveguide excitation system, photovoltaic conversion system, mechanical drive system and pc software. the minimum scanning resolution of the rls scanner was 5 mu m, and the maximum scanning range was 100 mm. the signal-to-noise ratio (snr) of rls was improved by the waveguide excitation mode. this device is capable of using a white light source as excitation source, thereby providing significant cost-savings. several kinds of microarrays including carbohydrate microarray, dna microarray and peptide microarray with gold nanoparticle (gnp) probes were used to demonstrate the detection performance of the device. detection limits of 1 ng ml(-1) and 100 pm in solution were obtained for concanavalin a (con a) and dna respectively. for the peptide on microarray spots, a detection limit of 100 fg was reported. the rls scanner showed at least one to two orders of magnitude more sensitive than that of commercial scanner. furthermore, taking advantage of the rls technique, the device was characterized by simple construction and low cost. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324296900005 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49646]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xing XF,Liu X,Li T,et al. Development of Light Waveguide Resonance Light-Scattering Scanner for Microarray Detection[J]. chinese journal of analytical chemistry,2013,41(9):1458-1462. |
| APA | Xing XF,Liu X,Li T,&Wang ZX.(2013).Development of Light Waveguide Resonance Light-Scattering Scanner for Microarray Detection.chinese journal of analytical chemistry,41(9),1458-1462. |
| MLA | Xing XF,et al."Development of Light Waveguide Resonance Light-Scattering Scanner for Microarray Detection".chinese journal of analytical chemistry 41.9(2013):1458-1462. |
入库方式： OAI收割
来源：长春应用化学研究所
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