Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay
文献类型：期刊论文
| 作者 | Li T ; Liu X ; Liu DJ ; Wang ZX |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:15页码:7033-7037 |
| 关键词 | GOLD NANOPARTICLE PROBES SIGNAL-TRANSDUCTION FLUORESCENCE PHOSPHORYLATION INHIBITION SPECIFICITY BIOSENSOR FORSKOLIN INSIGHTS BINDING |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang zx |
| 中文摘要 | in the present work, the activities of protein kinase a (pka) in cell lysates have been detected by a peptide microarray-based resonance light scattering assay with gold nanoparticle probes. highly sensitive detection of pica activity in 0.1 mu g total cell proteins of shg-44 cell lysate (corresponding to 200 cells) is achieved by a selected peptide substrate. the experimental results also demonstrate that the assay can be employed to evaluate expression levels of pka activity in different cell lines and chemical (e.g., forskolin)-mediated pka activity fluctuation in living cells. in addition, pka inhibition by the inhibitor (h89) is shown, suggesting the potential for screening pka inhibitors at the living cell level. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323014000011 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49685]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Liu X,Liu DJ,et al. Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay[J]. analytical chemistry,2013,85(15):7033-7037. |
| APA | Li T,Liu X,Liu DJ,&Wang ZX.(2013).Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay.analytical chemistry,85(15),7033-7037. |
| MLA | Li T,et al."Sensitive Detection of Protein Kinase A Activity in Cell Lysates by Peptide Microarray-Based Assay".analytical chemistry 85.15(2013):7033-7037. |
入库方式： OAI收割
来源：长春应用化学研究所
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