Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters
文献类型：期刊论文
| 作者 | Zhu SY ; Zhao XE ; Zhang W ; Liu ZY ; Qi WJ ; Anjuma S ; Xu GB |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2013 |
| 卷号 | 786页码:111-115 |
| 关键词 | AG NANOCLUSTERS QUANTITATIVE-DETERMINATION DNA DUPLEXES ASSAY NANOPARTICLES FLUOROPHORES PROBE |
| ISSN号 | 0003-2670 |
| 通讯作者 | xu gb |
| 中文摘要 | fluorescent silver nanoclusters stabilized by dna (dna-agncs) exhibit distinct response rates to thiol and disulfide. glutathione reductase can catalyze the reduction of the oxidized glutathione (gssg) quickly to reduced glutathione (gsh) in the presence of beta-nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt hydrate (nadph). consequently, dna-agncs can serve as a new fluorescent platform for assaying the glutathione reductase (gr) activity. this newly proposed assay has a high sensitivity and a good selectivity toward gr. the gr activity can be detected in the range of 0.2-2.0 mu ml(-1) with a minimum detectable concentration of 0.2 mu ml(-1). pepsin, lysozyme, trypsin, avidin, thrombin, myoglobin, and bsa have little effect on the fluorescence intensity of dna-agncs. the gr activity assay is successfully used to monitor the inhibition of gr activity by a typical inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea. (c) 2013 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320852300016 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49747]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu SY,Zhao XE,Zhang W,et al. Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters[J]. analytica chimica acta,2013,786:111-115. |
| APA | Zhu SY.,Zhao XE.,Zhang W.,Liu ZY.,Qi WJ.,...&Xu GB.(2013).Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters.analytica chimica acta,786,111-115. |
| MLA | Zhu SY,et al."Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters".analytica chimica acta 786(2013):111-115. |
入库方式： OAI收割
来源：长春应用化学研究所
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