Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(L-glutamic acid) and chitosan
文献类型：期刊论文
| 作者 | Zhang KX ; Zhang Y ; Yan SF ; Gong LL ; Wang J ; Chen X ; Cui L ; Yin JB |
| 刊名 | acta biomaterialia
 |
| 出版日期 | 2013 |
| 卷号 | 9期号:7页码:7276-7288 |
| 关键词 | MECHANICAL-PROPERTIES PORE-SIZE IN-VIVO TISSUE CHONDROCYTES |
| ISSN号 | 1742-7061 |
| 通讯作者 | chen xs |
| 中文摘要 | as a synthetic polypeptide water-soluble poly(l-glutamic acid) (plga) was designed to fabricate scaffolds for cartilage tissue engineering. chitosan (chi) has been employed as a physical cross-linking component in the construction of scaffolds. plga/chi scaffolds act as sponges with a swelling ratio of 760 +/- 45% (mass%), showing promising biocompatibility and biodegradation. autologous adipose-derived stem cells (ascs) were expanded and seeded on plga/chi scaffolds, asc/scaffold constructs were then subjected to chondrogenic induction in vitro for 2 weeks. the results showed that plga/chi scaffolds could effectively support asc adherence, proliferation and chondrogenic differentiation. the ascs/scaffold constructs were then transplanted to repair full thickness articular cartilage defects (4 mm in diameter, to the depth of subchondral bone) created in rabbit femur trochlea. histological observations found that articular defects were covered with newly formed cartilage 6 weeks post-implantation. after 12 weeks the regenerated cartilage had integrated well with the surrounding native cartilage and subchondral bone. toluidine blue and immunohistochemical staining confirmed similar accumulation of glycosaminoglycans and type ii collagen in engineered cartilage as in native cartilage 12 weeks post-implantation. the result was further supported by quantitative analysis of extracellular matrix deposition. the compressive modulus of the engineered cartilage increased significantly from 30% of that of normal cartilage at 6 weeks to 83% at 12 weeks. cyto-nanoindentation also showed analogous biomechanical behavior of the engineered cartilage to that of native cartilage. the results of the present study thus demonstrate the potentiality of plga/chi scaffolds in cartilage tissue engineering. (c) 2013 acts materialia inc. published by elsevier ltd. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320736700016 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49757]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang KX,Zhang Y,Yan SF,et al. Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(L-glutamic acid) and chitosan[J]. acta biomaterialia,2013,9(7):7276-7288. |
| APA | Zhang KX.,Zhang Y.,Yan SF.,Gong LL.,Wang J.,...&Yin JB.(2013).Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(L-glutamic acid) and chitosan.acta biomaterialia,9(7),7276-7288. |
| MLA | Zhang KX,et al."Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(L-glutamic acid) and chitosan".acta biomaterialia 9.7(2013):7276-7288. |
入库方式： OAI收割
来源：长春应用化学研究所
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