Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis. Part 2: Cations
文献类型：期刊论文
| 作者 | Redweik S ; Cianciulli C ; Hara M ; Xu YH ; Watzig H |
| 刊名 | electrophoresis
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:12页码:1812-1819 |
| 关键词 | BOVINE SERUM-ALBUMIN DYNAMIC LIGHT-SCATTERING BETA-LACTOGLOBULIN BINDING CONSTANTS METAL-IONS HYDRATION SHELL AGGREGATION NMR ADSORPTION UBIQUITIN |
| ISSN号 | 0173-0835 |
| 通讯作者 | watzig h |
| 中文摘要 | the influence of various cations as metal ions (barium, calcium, copper, magnesia, manganese, and nickel), pharmaceuticals (ephedrine, ethambutol, pilocarpine, and pirenzepine), arginine, and guanidine has been tested on bsa, -lactoglobulin, and ovalbumin. influences on proteins regarding changes in size, charge, or mass were of particular interest. ace proved to be a suitable method to investigate these effects. ace is able to observe slight but significant changes on proteins due to the excellent precision of the measurements. therefore, some unexpected behaviors of protein-ligand interactions were found. the protein charge becomes more negative under metal ion influence and some pharmaceutical cations. probably metal ions bound to the proteins form additional complexes with anions from the surrounding solution. furthermore, already bound cations could be displaced at the protein surface. both effects would change the overall charge of the ligand-protein complexes. in all studied cases, multiple-binding stoichiometries have been observed. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320385400014 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49825]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Redweik S,Cianciulli C,Hara M,et al. Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis. Part 2: Cations[J]. electrophoresis,2013,34(12):1812-1819. |
| APA | Redweik S,Cianciulli C,Hara M,Xu YH,&Watzig H.(2013).Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis. Part 2: Cations.electrophoresis,34(12),1812-1819. |
| MLA | Redweik S,et al."Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis. Part 2: Cations".electrophoresis 34.12(2013):1812-1819. |
入库方式： OAI收割
来源：长春应用化学研究所
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