Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA
文献类型：期刊论文
| 作者 | Qi RG ; Wu SH ; Wang Y ; Chen J ; Xie ZG ; Huang YB ; Jing XB |
| 刊名 | chinese journal of polymer science
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:6页码:912-923 |
| 关键词 | RNA INTERFERENCE IN-VIVO DELIVERY VEHICLES PLASMID DNA NANOPARTICLES PHARMACOKINETICS BIODISTRIBUTION METHACRYLATE) COPOLYMERS PROSPECTS |
| ISSN号 | 0256-7679 |
| 通讯作者 | jing xb |
| 中文摘要 | to further enhance the transfection efficiency of a micelleplex system based on monomethoxy poly(ethylene glycol)-block-poly(e >-caprolactone)-block-poly(l-lysine) (mpeg-b-pcl-b-pll), cholesterol (chol) moieties are attached to the e >-termini of pll segments to obtain mpeg-b-pcl-b-pll/chol. the structure and morphology of the copolymer are studied by h-1-nmr, tem and dls (dynamic light scattering). the cytotoxicity, cell uptake, endosomal release and mrna knockdown are studied by mtt (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, flow cytometry, clsm (confocal laser scanning microscopy) and rt-pcr (real-time polymerase chain reaction). the results show that compared to their precursor mpeg-b-pcl-b-pll, the cholesterol-grafted copolymer shows significantly lower toxicity, more rapid cellular endocytosis and endosome escape, and consequently displays enhanced sirna transfection efficiency even at a lower n/p ratio. these improvements are ascribed to enhanced interaction of the cholesterol moieties with both cellular membrane and endosomal membrane. moreover, effect of the pll block length is examined. the final conclusion is that long enough pll segments and incorporation of proper fraction of cholesterol onto the pll segments benefit the enhancement of sirna transfection efficiency. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317985500008 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49839]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qi RG,Wu SH,Wang Y,et al. Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA[J]. chinese journal of polymer science,2013,31(6):912-923. |
| APA | Qi RG.,Wu SH.,Wang Y.,Chen J.,Xie ZG.,...&Jing XB.(2013).Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA.chinese journal of polymer science,31(6),912-923. |
| MLA | Qi RG,et al."Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA".chinese journal of polymer science 31.6(2013):912-923. |
入库方式： OAI收割
来源：长春应用化学研究所
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