Determination of kinetic parameters and structureactivity relationships of ginsenosides as inhibitors of cyclin-dependent kinase 5/p25 using ultra-pressure liquid chromatography with triple quadrupole tandem mass spectrometry
文献类型：期刊论文
| 作者 | Chen N ; Yang HM ; Niu J ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2013 |
| 卷号 | 27期号:9页码:985-992 |
| 关键词 | PAIRED HELICAL FILAMENTS RECOMBINANT TAU-PROTEIN ALZHEIMERS-DISEASE ASSAYS CDK5 PHOSPHORYLATION SPECIFICITY ROSCOVITINE GINSENG INCLUSIONS |
| ISSN号 | 0951-4198 |
| 通讯作者 | liu sy |
| 中文摘要 | rationale cyclin-dependent kinase 5 (cdk5) is a serine/threonine kinase that is reported to play an important role in the pathogenesis of alzheimer's disease. ginsenosides have beneficial effects on alzheimer's disease in both in vivo and in vitro experiments, but the precise mechanisms are not yet entirely clear. methods in the present study, an ultrahigh-pressure liquid chromatography (uplc) and triple quadrupole mass spectrometry (tqms) assay was developed to study the activities of cdk5 for the first time. results the calibration curves showed a good linear behavior over the range 0.04m to 10m (y=0.934x+0.045, r2=0.995) with product phosphorylated peptide (pkptpkkakkv). the screening results suggested that the inhibition activities of ginsenosides are related to their chemical structures. conclusions the developed uplc/tqms-based method for determination of an inhibitor of cdk5/p25 is sensitive and reliable. the effect of ginsenosides on alzheimer's disease may be involved with the regulation of activities of cdk5/p25. copyright (c) 2013 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317980300010 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49857]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen N,Yang HM,Niu J,et al. Determination of kinetic parameters and structureactivity relationships of ginsenosides as inhibitors of cyclin-dependent kinase 5/p25 using ultra-pressure liquid chromatography with triple quadrupole tandem mass spectrometry[J]. rapid communications in mass spectrometry,2013,27(9):985-992. |
| APA | Chen N,Yang HM,Niu J,&Liu SY.(2013).Determination of kinetic parameters and structureactivity relationships of ginsenosides as inhibitors of cyclin-dependent kinase 5/p25 using ultra-pressure liquid chromatography with triple quadrupole tandem mass spectrometry.rapid communications in mass spectrometry,27(9),985-992. |
| MLA | Chen N,et al."Determination of kinetic parameters and structureactivity relationships of ginsenosides as inhibitors of cyclin-dependent kinase 5/p25 using ultra-pressure liquid chromatography with triple quadrupole tandem mass spectrometry".rapid communications in mass spectrometry 27.9(2013):985-992. |
入库方式： OAI收割
来源：长春应用化学研究所
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