Effects of Flavonoides from the Leaves of Acanthopanax on the Activity of CYP450 Isozymes in Rat Liver Microsomes by a UPLC-MS/MS and Cocktail Probe Substrates Method
文献类型：期刊论文
| 作者 | Bi YF ; Zhu HB ; Pi ZF ; Liu ZQ ; Song FR |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:5页码:1067-1071 |
| 关键词 | TRANSFORM MASS-SPECTROMETRY CYTOCHROME-P450 INHIBITION ACETYLCHOLINESTERASE BIOTRANSFORMATION METABOLITES |
| ISSN号 | 0251-0790 |
| 通讯作者 | song fr |
| 中文摘要 | effects of flavonoides including quercitrin, hyperoside and rutin from the leaves of acanthopanax on the activity of hepatic microsomal cyp450 isozymes(cyp1a2, cyp2c, cyp2e1, cyp2d and cyp3a) in rat liver microsomes were analyzed by a ultra performance liquid chromatography-tandem mass spectrometry (multiple reaction monitoring) [uplc-ms/ms(mrm)] combined with cocktail probe substrates method. the results showed that three compounds on the activities of some cyp450 isozymes were the inhibitory effects using rat liver microsomes, in which the ic50 values of quercitrin and hyperoside to the inhibition of rat microsomal cyp1a2 activity were 46. 53 and 49. 75 mu mol/l, respectively, the ic50 values of hyperoside and rutin for the inhibition of cyp2e1 activity were 99. 87 and 86. 36 mu mol/l, respectively. mechanism of inhibition experimental results show that three compounds on each cyp450 isozymes inhibiting ability was increased with the increasing of the preincubation time, therefore, the inhibitory effects was a mechanism-based inhibition. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000319546600007 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49861]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bi YF,Zhu HB,Pi ZF,et al. Effects of Flavonoides from the Leaves of Acanthopanax on the Activity of CYP450 Isozymes in Rat Liver Microsomes by a UPLC-MS/MS and Cocktail Probe Substrates Method[J]. chemical journal of chinese universities-chinese,2013,34(5):1067-1071. |
| APA | Bi YF,Zhu HB,Pi ZF,Liu ZQ,&Song FR.(2013).Effects of Flavonoides from the Leaves of Acanthopanax on the Activity of CYP450 Isozymes in Rat Liver Microsomes by a UPLC-MS/MS and Cocktail Probe Substrates Method.chemical journal of chinese universities-chinese,34(5),1067-1071. |
| MLA | Bi YF,et al."Effects of Flavonoides from the Leaves of Acanthopanax on the Activity of CYP450 Isozymes in Rat Liver Microsomes by a UPLC-MS/MS and Cocktail Probe Substrates Method".chemical journal of chinese universities-chinese 34.5(2013):1067-1071. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。