In Situ Nanoplasmonic Probing of Enzymatic Activity of Monolayer-Confined Glucose Oxidase on Colloidal Nanoparticles
文献类型：期刊论文
| 作者 | He HL ; Xu XL ; Wu HX ; Zhai YJ ; Jin YD |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:9页码:4546-4553 |
| 关键词 | GOLD NANOPARTICLES PLASMONIC NANOSENSORS PROTEIN CORONA QUANTUM DOTS NANOSHELLS INTERFACE BIOLOGY GROWTH SIZE NANOSTRUCTURES |
| ISSN号 | 0003-2700 |
| 通讯作者 | jin yd |
| 中文摘要 | in situ probing protein-particle interactions and activities of proteins on colloidal nanopartide (np) surfaces is a long-standing key challenge in understanding the nanobio interfaces and virtually important for a variety of biological and biomedical applications. the interactions of nps with proteins, for instance, are known to form np bioconjugates or protein coronas; protein surface immobilization and molecular layer-by-layer deposition techniques are widely used, but a clear understanding of the confinement effect on protein activity by molecular coating, at the monolayer level, remains poorly understood. we explore here a novel approach, using colloidal plasmonic nano-complexes coated with glucose oxidase (gox) as self-sensing nanoprobes for in situ optical probing of surface-confined enzymatic activity, which is at least 1-2 orders of magnitude more sensitive than standard colorimetric assays for detecting gox activity. we found that enzymatic activity of monolayer-confined gox on colloidal nps was significantly enhanced as compared with free gox (also proved by conformational changes from circular dichroism studies), with a low apparent michaelis-menten constant k-m of similar to 0.115 mm and high turnover k(cat)/k-m of similar to 8394 m-1.s(-1); compared with the "anchored-type" suspending gox , the outmost polyelectrolyte monolayer-protected sandwiched type gox exhibits significantly improved enzymatic activities toward higher temperatures and wider ph range. this finding is of fundamental important and instructive for safe use of such nanomaterials for bioapplications. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318756100050 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49867]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | He HL,Xu XL,Wu HX,et al. In Situ Nanoplasmonic Probing of Enzymatic Activity of Monolayer-Confined Glucose Oxidase on Colloidal Nanoparticles[J]. analytical chemistry,2013,85(9):4546-4553. |
| APA | He HL,Xu XL,Wu HX,Zhai YJ,&Jin YD.(2013).In Situ Nanoplasmonic Probing of Enzymatic Activity of Monolayer-Confined Glucose Oxidase on Colloidal Nanoparticles.analytical chemistry,85(9),4546-4553. |
| MLA | He HL,et al."In Situ Nanoplasmonic Probing of Enzymatic Activity of Monolayer-Confined Glucose Oxidase on Colloidal Nanoparticles".analytical chemistry 85.9(2013):4546-4553. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。