Kinetic Analysis of Interaction between Tumor Necrosis Factor and Soluble Receptors
文献类型：期刊论文
| 作者 | Yang F ; Yang XR |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 41期号:5页码:664-669 |
| 关键词 | OPTICAL BIOSENSOR LITERATURE RHEUMATOID-ARTHRITIS TNF-ALPHA PROTEIN |
| ISSN号 | 0253-3820 |
| 通讯作者 | yang xr |
| 中文摘要 | the kinetic analysis of interaction between tumor necrosis factor (tnf) and its two soluble extracellular receptors (sri/fc and srii/fc chimera) was carried out by using surface plasmon resonance (spr) technique. two immobilization strategies were used. for the first one, tnf was covalently immobilized on the cm5 sensor chip by amine coupling method, and the interaction between tnf and its soluble receptors were analyzed. for the second one, protein a was first covalently immobilized on the cm5 sensor chip by amine coupling method, and tnf sri/fc chimera or tnf srii/fc chimera was subsequently captured on the sensor chip through the specific interaction of their fc moiety with protein a. the kinetics of immobilized soluble receptors was studied by surface plasmon resonance. the experiment results showed that the kinetic parameters obtained from these two strategies correlated well. the affinity constant k-d of tnf with its receptors was around 10(-9) mol/l, indicating a higher affinity than that of the antibody. antigen interaction (k-d = 10(-5)-10(-7) mol/l) |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324166400005 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49873]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang F,Yang XR. Kinetic Analysis of Interaction between Tumor Necrosis Factor and Soluble Receptors[J]. chinese journal of analytical chemistry,2013,41(5):664-669. |
| APA | Yang F,&Yang XR.(2013).Kinetic Analysis of Interaction between Tumor Necrosis Factor and Soluble Receptors.chinese journal of analytical chemistry,41(5),664-669. |
| MLA | Yang F,et al."Kinetic Analysis of Interaction between Tumor Necrosis Factor and Soluble Receptors".chinese journal of analytical chemistry 41.5(2013):664-669. |
入库方式： OAI收割
来源：长春应用化学研究所
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