Preparation of Porous Silicon Substrate for Protein Microarray Fabrication by Double-Cell Electrochemical Etching Method
文献类型：期刊论文
| 作者 | Ji JM ; He XX ; Duan Q ; Wang ZX |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 41期号:5页码:698-703 |
| 关键词 | SILANIZATION SURFACE |
| ISSN号 | 0253-3820 |
| 通讯作者 | wang zx |
| 中文摘要 | uniformly porous silicon substrate with large surface area(12 mm. 58 mm) was prepared by simple double. cell electrochemical etching method. after functionalizing with epoxide groups, the porous silicon substrate can be employed to fabricate the protein microarray. well-known biomolecular recognition system (the interaction of mouse igg with rabbit anti-mouse igg antibody) was chosen to establish this new microarray format by proof of principle experiment. we found that the detection sensitivity of the mouse igg microarray was dependent on the preparing conditions of porous silicon substrate including etching current density, etching time, and concentration of hf solution. under the optimal conditions such as 500 ma/cm(2) of etching current density, 450 s of etching time and 25% hf solution, specific binding could be detected using rabbit anti. mouse igg antibody at concentrations as low as 10 mu g/l with a dynamic range of 0.32-10.0 mg/l. the experimental results demonstrate that the as-prepared porous silicon substrate could be used to fabricate the protein microarrays with high reaction capability. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324166400011 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49874]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ji JM,He XX,Duan Q,et al. Preparation of Porous Silicon Substrate for Protein Microarray Fabrication by Double-Cell Electrochemical Etching Method[J]. chinese journal of analytical chemistry,2013,41(5):698-703. |
| APA | Ji JM,He XX,Duan Q,&Wang ZX.(2013).Preparation of Porous Silicon Substrate for Protein Microarray Fabrication by Double-Cell Electrochemical Etching Method.chinese journal of analytical chemistry,41(5),698-703. |
| MLA | Ji JM,et al."Preparation of Porous Silicon Substrate for Protein Microarray Fabrication by Double-Cell Electrochemical Etching Method".chinese journal of analytical chemistry 41.5(2013):698-703. |
入库方式： OAI收割
来源：长春应用化学研究所
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