Signal-amplification detection of small molecules by use of Mg2+- dependent DNAzyme
文献类型：期刊论文
| 作者 | Guo ZJ ; Wang JH ; Wang EK |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 405期号:12页码:4051-4057 |
| 关键词 | FLUORESCENCE DETECTION OCHRATOXIN-A ELECTROCHEMICAL DETECTION ADENOSINE-TRIPHOSPHATE GOLD NANOPARTICLES DNA-ENZYME APTAMER ATP CATALYSIS TOXICITY |
| ISSN号 | 1618-2642 |
| 通讯作者 | wang jh |
| 中文摘要 | because small molecules can be beneficial or toxic in biology and the environment, specific and sensitive detection of small molecules is one of the most important objectives of the scientific community. in this study, new signal amplification assays for detection of small molecules based on mg2+-dependent dnazyme were developed. a cleavable dna substrate containing a ribonucleotide, the ends of which were labeled with black hole quencher (bhq) and 6-carboxyfluorescein (fam), was used for fluorescence detection. when the small molecule of interest is added to the assay solution, the mg2+-dependent dnazyme is activated, facilitating hybridization between the mg2+-dependent dnazyme and the dna substrate. binding of the substrate to the dnazyme structure results in hydrolytic cleavage of the substrate in the presence of mg2+ ions. the fluorescence signal was amplified by continuous cleavage of the enzyme substrate. ochratoxin a (ota) and adenosine triphosphate (atp) were used as model analytes in these experiments. this method can detect ota specifically with a detection limit as low as 140 pmol l-1 and detect atp specifically with a detection limit as low as 13 nmol l-1. moreover, this method is potentially extendable to detection of other small molecules which are able to dissociate the aptamer from the dnazyme, leading to activation of the dnazyme. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317643900018 |
| 公开日期 | 2014-04-15 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49882]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Guo ZJ,Wang JH,Wang EK. Signal-amplification detection of small molecules by use of Mg2+- dependent DNAzyme[J]. analytical and bioanalytical chemistry,2013,405(12):4051-4057. |
| APA | Guo ZJ,Wang JH,&Wang EK.(2013).Signal-amplification detection of small molecules by use of Mg2+- dependent DNAzyme.analytical and bioanalytical chemistry,405(12),4051-4057. |
| MLA | Guo ZJ,et al."Signal-amplification detection of small molecules by use of Mg2+- dependent DNAzyme".analytical and bioanalytical chemistry 405.12(2013):4051-4057. |
入库方式： OAI收割
来源：长春应用化学研究所
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