A Serum-Tolerant Hydroxyl-Modified Polyethylenimine as Versatile Carriers of pDNA/siRNA
文献类型：期刊论文
| 作者 | Dong X ; Lin L ; Chen J ; Guo ZP ; Tian HY ; Li YC ; Wei Y ; Chen XS |
| 刊名 | macromolecular bioscience
 |
| 出版日期 | 2013 |
| 卷号 | 13期号:4页码:512-522 |
| 关键词 | GENE DELIVERY SIRNA DELIVERY DNA DELIVERY HYPERBRANCHED PEI COPOLYMERS TRANSFECTION VECTORS CELLS DERIVATIVES THERAPY |
| ISSN号 | 1616-5187 |
| 通讯作者 | tian hy |
| 中文摘要 | the polyethylenimine (pei) derivatives (ptn) are prepared by treating pei25k with tris(hydroxymethyl) acrylamidomethane via the michael addition. these ptns can effectively condense nucleic acids into nanosized particles with positive surface charges. the ptns show lower cytotoxicity and better serum-resistant capacity than pei25k. specially, the transfection efficiency of pt26/dna is 29-fold higher than that of pei25k in hela cells in serum-containing medium. the ptn/sirna complexes show superior knockdown effect in ct26 cells in serum-containing medium. in addition, flow cytometry analysis shows that the ptns can efficiently mediate the entry of nucleic acids into the cell. thus, ptns are potentially applicable as non-viral carriers of nucleic acids and warrant further development for use in gene therapy. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000318043400013 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49932]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Dong X,Lin L,Chen J,et al. A Serum-Tolerant Hydroxyl-Modified Polyethylenimine as Versatile Carriers of pDNA/siRNA[J]. macromolecular bioscience,2013,13(4):512-522. |
| APA | Dong X.,Lin L.,Chen J.,Guo ZP.,Tian HY.,...&Chen XS.(2013).A Serum-Tolerant Hydroxyl-Modified Polyethylenimine as Versatile Carriers of pDNA/siRNA.macromolecular bioscience,13(4),512-522. |
| MLA | Dong X,et al."A Serum-Tolerant Hydroxyl-Modified Polyethylenimine as Versatile Carriers of pDNA/siRNA".macromolecular bioscience 13.4(2013):512-522. |
入库方式： OAI收割
来源：长春应用化学研究所
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