Newborn screening of phenylketonuria using direct analysis in real time (DART) mass spectrometry
文献类型：期刊论文
| 作者 | Wang CY ; Zhu HB ; Cai ZW ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 405期号:10页码:3159-3164 |
| 关键词 | LIQUID-CHROMATOGRAPHIC ANALYSIS AMINO-ACIDS DRIED BLOOD PHYSIOLOGICAL SAMPLES PHENYLALANINE QUANTIFICATION BIOANALYSIS DIAGNOSIS MATRICES TYROSINE |
| ISSN号 | 1618-2642 |
| 通讯作者 | song fr |
| 中文摘要 | phenylketonuria (pku) is commonly included in the newborn screening panel of most countries, with various techniques being used for quantification of l-phenylalanine (phe). to diagnose pku as early as possible in newborn screening, a rapid and simple method of analysis was developed. using direct analysis in real time (dart) ionization coupled with triple-quadrupole tandem mass spectrometry (tq-ms/ms) and with use of a 12 dip-it tip scanner autosampler in positive ion mode, we analyzed dried blood spot (dbs) samples from pku newborns. the concentration of phe was determined using multiple reaction monitoring mode with the nondeuterated internal standard n,n-dimethylphenylalanine. the results of the analysis of dbs samples from newborns indicated that the dart-tq-ms/ms method is fast, accurate, and reproducible. the results prove that this assay as a newborn screen for pku can be performed in 18 s per sample for the quantification of phe in dbs samples. dart-tq-ms/ms analysis of the phe concentration in dbs samples allowed us to screen newborns for pku. this innovative protocol is rapid and can be effectively applied on a routine basis to analyze a large number of samples in pku newborn screening and pku patient monitoring. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000316338700015 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49949]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang CY,Zhu HB,Cai ZW,et al. Newborn screening of phenylketonuria using direct analysis in real time (DART) mass spectrometry[J]. analytical and bioanalytical chemistry,2013,405(10):3159-3164. |
| APA | Wang CY,Zhu HB,Cai ZW,Song FR,Liu ZQ,&Liu SY.(2013).Newborn screening of phenylketonuria using direct analysis in real time (DART) mass spectrometry.analytical and bioanalytical chemistry,405(10),3159-3164. |
| MLA | Wang CY,et al."Newborn screening of phenylketonuria using direct analysis in real time (DART) mass spectrometry".analytical and bioanalytical chemistry 405.10(2013):3159-3164. |
入库方式： OAI收割
来源：长春应用化学研究所
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