Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor
文献类型：期刊论文
| 作者 | Liu JY ; Qin YN ; Li D ; Wang TS ; Liu YQ ; Wang J ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2013 |
| 卷号 | 41页码:436-441 |
| 关键词 | ESCHERICHIA-COLI O157-H7 SELF-ASSEMBLED MONOLAYER K562 LEUKEMIA-CELLS GLUCOSE-OXIDASE NANOPARTICLES BIOSENSORS SENSORS IMMOBILIZATION PROLIFERATION IMMUNOSENSOR |
| ISSN号 | 0956-5663 |
| 通讯作者 | liu yq |
| 中文摘要 | electrochemical methods have attracted considerable attention for developing cytosensing system since they can decrease the cost and time requirement for cell detection with simple instrumentation. herein, a label-free electrochemical cytosensor with surface-confined ferrocene as signal indicator was developed for highly sensitive and selective detection of cancer cell. with layer-by-layer (lbl) self-assembly technique, positively charged poly(ethylene imine) functionalized with ferrocene (fc-pei) and negatively charged single-wall carbon nanotubes (swnts) were alternately assembled on 3-mercaptopropionic acid (mpa) modified gold substrate. folic acid (fa) was covalently bonded onto swnts surface to specifically recognize cancer cells according to the high affinity of fa for folate receptor (fr) on cellular surface. the developed cytosensor presented high sensitivity and selectivity for the detection of human cervical carcinoma (hela) cell. by using fast-response differential pulse voltammetry (dpv) method, a wide detection range from 10 to 10(6) cells/ml with a detection limit as low as 10 cells/ml was reached even in the presence of a large amount of non-cancerous cells. (c) 2012 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314191300064 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49974]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu JY,Qin YN,Li D,et al. Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor[J]. biosensors & bioelectronics,2013,41:436-441. |
| APA | Liu JY.,Qin YN.,Li D.,Wang TS.,Liu YQ.,...&Wang EK.(2013).Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor.biosensors & bioelectronics,41,436-441. |
| MLA | Liu JY,et al."Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor".biosensors & bioelectronics 41(2013):436-441. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。