G-quadruplex-based ultrasensitive and selective detection of histidine and cysteine
文献类型：期刊论文
| 作者 | Li HL ; Liu JY ; Fang YX ; Qin YA ; Xu SL ; Liu YQ ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2013 |
| 卷号 | 41页码:563-568 |
| 关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY CAPILLARY-ZONE-ELECTROPHORESIS MASS-SPECTROMETRY AMINO-ACIDS ELECTROCHEMICAL DETECTION FLUORESCENCE DETECTION VOLTAMMETRIC DETERMINATION PRECOLUMN DERIVATIZATION AMPEROMETRIC DETECTION POTASSIUM-ION |
| ISSN号 | 0956-5663 |
| 通讯作者 | liu yq |
| 中文摘要 | histidine and cysteine detection is critically important since an abnormal level of histidine or cysteine is an indicator for many diseases. in this paper, we demonstrated a novel label-free, g-quadruplex-based approach for simultaneous detection of histidine and cysteine. the present assay is based on the highly specific interaction among amino acids (histidine or cysteine), cu2+ and nmm/g-4 (nmm: n-methylmesoporphyrin ix; g-4: g-quadruplex). the fluorescence intensity of nmm was dramatically enhanced in the presence of g-quadruplex formed from 24gt, which can be effectively quenched by cupric ion (cu2+) due to the chelation of cu2+ by nmm as well as the unfolding of g-quadruplex by cu2+. the presence of histidine or cysteine will disturb the interaction between cu2+ and nmm/g-4 because of the strong binding affinity of cu2+ to the imidazole group of histidine or the interaction of cu2+ with thiol group in cysteine, leading to distinct fluorescence emission intensity. high selectivity is conferred by the use of cysteine-masking agent n-ethylmaleimide (nem), which helps to discriminate histidine from cysteine. at last, a novel and simple approach was developed to determine each precise concentration of histidine and cysteine according to the different response of the system with and without nem. importantly, histidine can be also detected even in the presence of a large amount of other amino acids. a detection limit as low as 3 nm for histidine and 5 nm for cysteine was obtained by practical measurement rather than conventional calculation (s/n=3), confirming the high sensitivity of the present approach. meanwhile, this sensing protocol can determine histidine and cysteine in diluted biological samples such as urine, exhibiting great potential to meet the need of practical application. (c) 2012 published by elsevier b.v. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314191300084 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49975]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li HL,Liu JY,Fang YX,et al. G-quadruplex-based ultrasensitive and selective detection of histidine and cysteine[J]. biosensors & bioelectronics,2013,41:563-568. |
| APA | Li HL.,Liu JY.,Fang YX.,Qin YA.,Xu SL.,...&Wang EK.(2013).G-quadruplex-based ultrasensitive and selective detection of histidine and cysteine.biosensors & bioelectronics,41,563-568. |
| MLA | Li HL,et al."G-quadruplex-based ultrasensitive and selective detection of histidine and cysteine".biosensors & bioelectronics 41(2013):563-568. |
入库方式： OAI收割
来源：长春应用化学研究所
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