In Situ Formation of Metal Coordination Polymer: A Strategy for Fluorescence Turn-On Assay of Acetylcholinesterase Activity and Inhibitor Screening
文献类型：期刊论文
| 作者 | Liao DL ; Chen J ; Zhou HP ; Wang Y ; Li YX ; Yu C |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:5页码:2667-2672 |
| 关键词 | CONTINUOUS FLUOROMETRIC ASSAY AGGREGATION-INDUCED EMISSION ALZHEIMERS-DISEASE PERYLENE PROBE TERPYRIDYL COMPLEXES TORPEDO-CALIFORNICA ENZYME ENSEMBLE PROTEIN |
| ISSN号 | 0003-2700 |
| 通讯作者 | yu c |
| 中文摘要 | a novel method for the sensing of acetylcholinesterase (ache) activity and inhibitor screening based on the formation of metal coordination polymer has been developed. acetylthiocholine (atch) was selected as the substrate. in the presence of ache, atch was hydrolyzed to thiocholine and acetate. thiocholine interacted with ag(i) to form a metal coordination polymer. a positively charged perylene probe (probe 1) was employed. the fluorescence of probe 1 was very efficiently quenched by a polyanion [pvs, poly(vinyl sulfonate)]. in the presence of acetylcholinesterase, the positively charged metal coordination polymer newly formed in situ would interact with pvs, probe 1 monomer molecules were released, and a turn on fluorescence signal was detected. the assay is highly sensitive, a limit of detection of 0.04 mu/ml ache was obtained. the assay is also highly selective, a number of potential interference proteins (enzymes) were tested, and none of them show noticeable interference. sensing of ache inhibitor was also demonstrated. our assay is fairly simple and inexpensive. we envision that it could be used for the sensitive detection of other hydrolytic enzyme activities with properly selected substrates and for the screening of potential inhibitor drugs. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000317031600019 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/49989]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liao DL,Chen J,Zhou HP,et al. In Situ Formation of Metal Coordination Polymer: A Strategy for Fluorescence Turn-On Assay of Acetylcholinesterase Activity and Inhibitor Screening[J]. analytical chemistry,2013,85(5):2667-2672. |
| APA | Liao DL,Chen J,Zhou HP,Wang Y,Li YX,&Yu C.(2013).In Situ Formation of Metal Coordination Polymer: A Strategy for Fluorescence Turn-On Assay of Acetylcholinesterase Activity and Inhibitor Screening.analytical chemistry,85(5),2667-2672. |
| MLA | Liao DL,et al."In Situ Formation of Metal Coordination Polymer: A Strategy for Fluorescence Turn-On Assay of Acetylcholinesterase Activity and Inhibitor Screening".analytical chemistry 85.5(2013):2667-2672. |
入库方式： OAI收割
来源：长春应用化学研究所
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