G-quadruplex-Based Fluorescent Assay of S1 Nuclease Activity and K+
文献类型：期刊论文
| 作者 | Zhou ZX ; Zhu JB ; Zhang LB ; Du Y ; Dong SJ ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013 |
| 卷号 | 85期号:4页码:2431-2435 |
| 关键词 | LINKED-IMMUNOSORBENT-ASSAY POTASSIUM-ION DETECTION LABEL-FREE CARBON NANOTUBES GRAPHENE OXIDE DNA CLEAVAGE RECOMBINATION AMPLIFICATION ENDONUCLEASE |
| ISSN号 | 0003-2700 |
| 通讯作者 | dong sj |
| 中文摘要 | endonuclease plays an important role in many biological processes, and an assay of endonuclease activity is of great significance. however, traditional methods for the assay of endonuclease activity have undesirable limitations, such as high cost, dna-consuming and laboriousness. in the present work, a g-quadruplex-based, fluorescent assay of endonuclease activity has been developed with protoporphyrin ix (ppix) as a signal reporter. si nuclease, a single strand dna (ssdna)-specific endonudease, is employed as model system. in the "on" state, g-quadruplex dna can greatly enhance the fluorescence of ppix. however, if si nuclease could cleave g-quadruplex dna into small fragments, there would be no formation of g-quadruplexes, accompanied by low emission response of ppix this fluorescent discrimination before or after digestion by nuclease can be used to monitor the activity of si nuclease. this assay is simple in design and offers a convenient protocol for homogeneous, rapid and high-throughput detection. in addition, the proposed strategy avoids complicated covalent modifications or chemical labeling, and thus offers advantages of simplicity and cost efficiency. more importantly, k+ is found to well inhibit the activity of si nuclease when using certain g-quadruplex dna as substrate, and thus this system is further used for turn-on detection of k+ si nuclease is critical in the detection of k+ since it helps to reduce the background signal. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000315326900066 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50030]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou ZX,Zhu JB,Zhang LB,et al. G-quadruplex-Based Fluorescent Assay of S1 Nuclease Activity and K+[J]. analytical chemistry,2013,85(4):2431-2435. |
| APA | Zhou ZX,Zhu JB,Zhang LB,Du Y,Dong SJ,&Wang EK.(2013).G-quadruplex-Based Fluorescent Assay of S1 Nuclease Activity and K+.analytical chemistry,85(4),2431-2435. |
| MLA | Zhou ZX,et al."G-quadruplex-Based Fluorescent Assay of S1 Nuclease Activity and K+".analytical chemistry 85.4(2013):2431-2435. |
入库方式： OAI收割
来源：长春应用化学研究所
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