Study of the interactions between the key spore coat morphogenetic proteins CotE and SpoVID
文献类型：期刊论文
| 作者 | Qiao HY ; Krajcikova D ; Xing CY ; Lu BP ; Hao JH ; Ke X ; Wang HD ; Barak I ; Tang JL |
| 刊名 | journal of structural biology
 |
| 出版日期 | 2013 |
| 卷号 | 181期号:2页码:128-135 |
| 关键词 | BACILLUS-SUBTILIS SPORE ATOMIC-FORCE MICROSCOPY SPORULATION GENE RESISTANCE ENDOSPORE LOCALIZATION CONSTRUCTION SPECTROSCOPY EXPRESSION SURFACE |
| ISSN号 | 1047-8477 |
| 通讯作者 | barak i |
| 中文摘要 | the capability of bacillus subtilis spores to withstand extreme environmental conditions is thought to be conferred especially by their outermost proteinaceous protective layer, called the spore coat. of the over 70 proteins that form the spore coat, only a small subset of them affect its morphogenesis, they are referred to as morphogenetic proteins. in this study we investigated the interaction between two spore coat morphogenetic proteins spovid and cote. spovid is involved in the process of spore surface encirclement by individual coat proteins, these include cote, which controls the assembly of the outer coat layer. both proteins were proposed to be recruited to a common protein scaffold, but their direct association has not been previously shown. here we studied the interactions between cote and spovid in vitro for the first time by using molecule recognition force spectroscopy, which allows the detection of pico-newton forces between conjugated biological pairs and also facilitates the investigation of dynamic processes. the most probable cote-cote unbinding force was 49.4 +/- 0.1 pn at a loading rate of 3.16 x 10(3) pn/s while that of spovid-cote was 26.5 +/- 0.6 pn at a loading rate of 7.8 x 10(2) pn/s. we further analyzed the interactions with the bacterial two hybrid system and pull-down experiments, which also indicate that spovid interacts directly with cote. in combination with the previously identified direct contacts among spoiva, spovid and safa, our data imply that the physical association of key morphogenetic proteins forms a basic skeleton where other coat proteins could be attached. (c) 2012 elsevier inc. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314627600005 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50066]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qiao HY,Krajcikova D,Xing CY,et al. Study of the interactions between the key spore coat morphogenetic proteins CotE and SpoVID[J]. journal of structural biology,2013,181(2):128-135. |
| APA | Qiao HY.,Krajcikova D.,Xing CY.,Lu BP.,Hao JH.,...&Tang JL.(2013).Study of the interactions between the key spore coat morphogenetic proteins CotE and SpoVID.journal of structural biology,181(2),128-135. |
| MLA | Qiao HY,et al."Study of the interactions between the key spore coat morphogenetic proteins CotE and SpoVID".journal of structural biology 181.2(2013):128-135. |
入库方式： OAI收割
来源：长春应用化学研究所
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