Second-tier test for quantification of underivatized amino acids in dry blood spot for metabolic diseases in newborn screening
文献类型：期刊论文
| 作者 | Wang CY ; Zhu HB ; Zhang WY ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | amino acids
 |
| 出版日期 | 2013 |
| 卷号 | 44期号:2页码:661-671 |
| 关键词 | TANDEM MASS-SPECTROMETRY INHERITED DISORDERS BIOLOGICAL INTEREST RAPID DIAGNOSIS MS/MS ANALYSIS INBORN-ERRORS ESI-MS/MS TOOL PHENYLKETONURIA PHENYLALANINE |
| ISSN号 | 0939-4451 |
| 通讯作者 | song fr |
| 中文摘要 | the quantitative analysis of amino acids (aas) in single dry blood spot (dbs) samples is an important issue for metabolic diseases as a second-tier test in newborn screening. an analytical method for quantifying underivatized aas in dbs was developed by using liquid chromatography coupled with tandem mass spectrometry (lc-ms/ms). the sample preparation in this method is simple and ion-pairing agent is not used in the mobile phase that could avoid ion suppression, which happens in mass spectrometry and avoids damage to the column. through chromatographic separation, some isomeric compounds could be identified and quantified, which cannot be solved through only appropriate multiple reactions monitoring transitions by ms/ms. the concentrations of the different aas were determined using non-deuterated internal standard. all calibration curves showed excellent linearity within test ranges. for most of the amino acids the accuracy of extraction recovery was between 85.3 and 115 %, and the precision of relative standard deviation was < 7.0 %. the 35 aas could be identified in dbs specimens by the developed lc-ms/ms method in 17-19 min, and eventually 24 aas in dbs were quantified. the results of the present study prove that this method as a second-tier test in newborn screening for metabolic diseases could be performed by the quantification of free aas in dbs using the lc-ms/ms method. the assay has advantages of high sensitive, specific, and inexpensive merits because non-deuterated internal standard and acetic acid instead of ion-pairing agent in mobile phase are used in this protocol. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000313794600035 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50068]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang CY,Zhu HB,Zhang WY,et al. Second-tier test for quantification of underivatized amino acids in dry blood spot for metabolic diseases in newborn screening[J]. amino acids,2013,44(2):661-671. |
| APA | Wang CY,Zhu HB,Zhang WY,Song FR,Liu ZQ,&Liu SY.(2013).Second-tier test for quantification of underivatized amino acids in dry blood spot for metabolic diseases in newborn screening.amino acids,44(2),661-671. |
| MLA | Wang CY,et al."Second-tier test for quantification of underivatized amino acids in dry blood spot for metabolic diseases in newborn screening".amino acids 44.2(2013):661-671. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。