Fingerprint analysis of Radix Aconiti using ultra-performance liquid chromatography-electrospray ionization/ tandem mass spectrometry (UPLC-ESI/MSn) combined with stoichiometry
文献类型：期刊论文
| 作者 | Zhu HB ; Wang CY ; Qi Y ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | talanta
 |
| 出版日期 | 2013 |
| 卷号 | 103页码:56-65 |
| 关键词 | DIESTER-DITERPENOID ALKALOIDS QUANTITATIVE-ANALYSIS CHEMICAL-COMPOSITION PATTERN-RECOGNITION GAS-CHROMATOGRAPHY HOUTTUYNIA-CORDATA QUALITY ASSESSMENT HERBAL MEDICINES CLUSTER-ANALYSIS ESI-MS |
| ISSN号 | 0039-9140 |
| 通讯作者 | song fr |
| 中文摘要 | a fingerprinting approach was developed by means of uplc-esi/msn (ultra-performance liquid chromatography-electrospray ionization/mass spectrometry) for the quality control of processed radix aconiti, a widely used toxic traditional herbal medicine. the present fingerprinting approach was based on the two processing methods recorded in chinese pharmacopoeia for the purpose of reducing the toxicity and ensuring the clinical therapeutic efficacy. similarity evaluation, hierarchical cluster analysis and principal component analysis were performed to evaluate the similarity and variation of the samples. the results showed that the well processed, unqualified processed and the raw radix aconiti could be clustered reasonably corresponding to the contents of their constituents. the loading plot shows that the main chemical markers having the most influence on the discrimination amongst the qualified and unqualified samples were mainly some monoester diterpenoid aconitines and diester diterpenoid aconitines. finally, the uplc-uv and uplc-esi/msn characteristic fingerprints were established according to the well processed and purchased qualified samples. at the same time, a complementary quantification method of six aconitine-type alkaloids was developed using uplc-uv and uplc-esi/ms. the average recovery of the monoester diterpenoid aconitines was 95.4-99.1% and the average recovery of the diester diterpenoid aconitines was 103-112%. the proposed combined quantification method by uplc-uv and uplc-esi/ms allows the samples analyzed in a wide concentration range. therefore, the established fingerprinting approach in combination with chemometric analysis provides a flexible and reliable method for quality assessment of toxic herbal medicine. (c) 2012 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314010600009 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50098]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu HB,Wang CY,Qi Y,et al. Fingerprint analysis of Radix Aconiti using ultra-performance liquid chromatography-electrospray ionization/ tandem mass spectrometry (UPLC-ESI/MSn) combined with stoichiometry[J]. talanta,2013,103:56-65. |
| APA | Zhu HB,Wang CY,Qi Y,Song FR,Liu ZQ,&Liu SY.(2013).Fingerprint analysis of Radix Aconiti using ultra-performance liquid chromatography-electrospray ionization/ tandem mass spectrometry (UPLC-ESI/MSn) combined with stoichiometry.talanta,103,56-65. |
| MLA | Zhu HB,et al."Fingerprint analysis of Radix Aconiti using ultra-performance liquid chromatography-electrospray ionization/ tandem mass spectrometry (UPLC-ESI/MSn) combined with stoichiometry".talanta 103(2013):56-65. |
入库方式： OAI收割
来源：长春应用化学研究所
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