Study on noncovalent complexes of alkaloids with DNA duplex using electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Ma L ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2013 |
| 卷号 | 27期号:1页码:51-58 |
| 关键词 | DOUBLE-STRANDED OLIGODEOXYNUCLEOTIDES CYTOTOXIC PROTOBERBERINE ALKALOIDS ISOQUINOLINE ALKALOIDS NATURAL-PRODUCTS PROMOTER DNA MINOR-GROOVE IN-VITRO BINDING BERBERINE MODE |
| ISSN号 | 0951-4198 |
| 通讯作者 | liu zq |
| 中文摘要 | rationale dna is an important molecular target in modern medicine research. some dna-binding ligands have been used to treat numerous diseases. therefore, understanding the interactions of different ligands with dna and looking for new dna agents are necessary to develop new drugs. methods electrospray ionization mass spectrometry (esi-ms) in the negative ion mode was used to screen the noncovalent complexes between 11 alkaloids with double helix oligonucleotides at molar ratios 1:1 to 1:4. the relative binding affinities based on the fraction of bound dna and sequence selectivities of alkaloids towards the duplex were also investigated by esi-ms. moreover, tandem mass spectrometry of 5-charged complex ions was used to try to determine dna-binding modes. results six alkaloids showed complexation with the selected dna duplex, i.e., berberine, coptisine, peimine, aconitine, oxysophoridine and cytisine. they showed their binding affinities with d(aactcccggcacac/gtgtgccgggagtt) in the order of berberine > coptisine > peimine > aconitine, oxysophoridine > cytosine; additional experiments involving collision energy proved this result. sequence selectivities were not apparent for coptisine, peimine, aconitine and oxysophoridine with four dna duplexes. the complexes containing berberine and coptisine underwent the predominant loss of the g base. however, for complexes containing the other four alkaloids, they dissociated via the loss of neutral drug. the results confirmed that they may have different binding modes. conclusions according to experiment data and structural information, the binding mode of individual drugs with dna was speculated. it was noted that the bindings of alkaloids peimine, aconitine and oxysophoridine with dna are discovered firstly. this may give a clue to design duplex-binding ligands and be helpful for understanding biological activities of these alkaloids. copyright (c) 2012 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312536700004 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50101]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ma L,Song FR,Liu ZQ,et al. Study on noncovalent complexes of alkaloids with DNA duplex using electrospray ionization mass spectrometry[J]. rapid communications in mass spectrometry,2013,27(1):51-58. |
| APA | Ma L,Song FR,Liu ZQ,&Liu SY.(2013).Study on noncovalent complexes of alkaloids with DNA duplex using electrospray ionization mass spectrometry.rapid communications in mass spectrometry,27(1),51-58. |
| MLA | Ma L,et al."Study on noncovalent complexes of alkaloids with DNA duplex using electrospray ionization mass spectrometry".rapid communications in mass spectrometry 27.1(2013):51-58. |
入库方式： OAI收割
来源：长春应用化学研究所
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