Human cytochrome P450 3A4 and a carbon nanofiber modified film electrode as a platform for the simple evaluation of drug metabolism and inhibition reactions
文献类型：期刊论文
| 作者 | Xue Q ; Kato D ; Kamata T ; Guo QH ; You TY ; Niwa O |
| 刊名 | analyst
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:21页码:6463-6468 |
| 关键词 | ELECTROCHEMICALLY-DRIVEN GLUCOSE BIOSENSOR CYP3A4 NANOPARTICLES PERFORMANCE COMPOSITES MICROSOMES NANOTUBES OXIDASE |
| ISSN号 | 0003-2654 |
| 通讯作者 | niwa o |
| 中文摘要 | electrochemical biosensors consisting of cytochrome p450 enzyme modified electrodes have been developed to provide a simple method for screening the metabolism of a drug and its inhibitor. here, we report a very simple electrochemically driven biosensor for detecting drug metabolism and its inhibition based on cytochrome p450 3a4 (cyp3a4) and a carbon nanofiber (cnf) modified film electrode without any other modified layers such as mediator films. direct electron transfer (det) between cyp3a4 and cnfs was observed at a formal potential of 0.302 v. the electrocatalytic reduction current increased with the addition of drugs including testosterone and quinidine. in contrast, the reduction current was greatly suppressed in the presence of ketoconazole, which is a cyp3a4 inhibitor. cnfs with high conductivity, a large surface area and sufficient edge planes provide a suitable microenvironment for achieving excellent det and biocatalysis properties, which could not be observed when we used other carbon materials such as carbon nanotube (cnt) and carbon black (cb) modified electrodes, indicating that our system is promising as a new bioelectronic platform for electrochemical biosensing. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000325366700036 |
| 公开日期 | 2014-04-16 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50172]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xue Q,Kato D,Kamata T,et al. Human cytochrome P450 3A4 and a carbon nanofiber modified film electrode as a platform for the simple evaluation of drug metabolism and inhibition reactions[J]. analyst,2013,138(21):6463-6468. |
| APA | Xue Q,Kato D,Kamata T,Guo QH,You TY,&Niwa O.(2013).Human cytochrome P450 3A4 and a carbon nanofiber modified film electrode as a platform for the simple evaluation of drug metabolism and inhibition reactions.analyst,138(21),6463-6468. |
| MLA | Xue Q,et al."Human cytochrome P450 3A4 and a carbon nanofiber modified film electrode as a platform for the simple evaluation of drug metabolism and inhibition reactions".analyst 138.21(2013):6463-6468. |
入库方式： OAI收割
来源：长春应用化学研究所
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