Ceria-based nanocomposites for the enrichment and identification of phosphopeptides
文献类型：期刊论文
| 作者 | Fatima B ; Najam-ul-Haq M ; Jabeen F ; Majeed S ; Ashiq MN ; Musharraf SG ; Shad BA ; Xu GB |
| 刊名 | analyst
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:17页码:5059-5067 |
| 关键词 | MASS-SPECTROMETRIC ANALYSIS PHASE LIQUID-CHROMATOGRAPHY TIN DIOXIDE MICROSPHERES PROTEIN-PHOSPHORYLATION ZIRCONIA COMPOSITE MS/MS ANALYSIS PHOSPHOPROTEOMICS OXIDE MELDI PERFORMANCE |
| ISSN号 | 0003-2654 |
| 通讯作者 | najam-ul-haq m |
| 中文摘要 | nanocomposites are given preference over the individual materials due to the combined properties of the components involved. ceria has a high efficiency in phosphopeptide enrichment as well as in dephosphorylation. iron oxide and tin oxide are chosen as counter metal oxides to synthesize the ceria nanocomposites using a co-precipitation method. the nanocomposites are characterized by fourier-transform infrared spectroscopy (ft-ir) and scanning electron microscopy (sem). tryptic beta-casein digest shows the feasibility of phosphopeptide enrichment by the two nanocomposites. selectivity studies show their performance in comparison to ceria. being more selective in the extended mass range, both nanocomposites are applied to spiked human serum and non-fat milk digest. the ceria nanocomposites are also capable of being used as material-enhanced laser desorption/ionization (meldi) carrier/affinity materials for real biological samples with varying degrees of complexity. the enriched content is analyzed by maldi-tof ms. all the phosphopeptides in all variants of casein are identified. the sequence coverage of caseins is also interpreted. nanocomposites thus offer a high selectivity and sensitivity, which make them promising materials for biomarker discoveries and the identification of phosphorylation pathways for new post translational modifications (ptms). |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000322389600048 |
| 公开日期 | 2014-04-18 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50226]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Fatima B,Najam-ul-Haq M,Jabeen F,et al. Ceria-based nanocomposites for the enrichment and identification of phosphopeptides[J]. analyst,2013,138(17):5059-5067. |
| APA | Fatima B.,Najam-ul-Haq M.,Jabeen F.,Majeed S.,Ashiq MN.,...&Xu GB.(2013).Ceria-based nanocomposites for the enrichment and identification of phosphopeptides.analyst,138(17),5059-5067. |
| MLA | Fatima B,et al."Ceria-based nanocomposites for the enrichment and identification of phosphopeptides".analyst 138.17(2013):5059-5067. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。