Application of MALDI-TOF-Mass Spectrometry to Proteome Analysis Using Stain-Free Gel Electrophoresis
文献类型：期刊论文
| 作者 | Susnea I ; Bernevic B ; Wicke M ; Ma L ; Liu SY ; Schellander K ; Przybylski M |
| 刊名 | applications of maldi-tof spectroscopy
 |
| 出版日期 | 2013 |
| 卷号 | 331页码:37-54 |
| 关键词 | LASER DESORPTION IONIZATION POLYACRYLAMIDE-GELS NATIVE FLUORESCENCE PROTEINS IDENTIFICATION DEGRADATION ULTRAVIOLET COMPATIBILITY PEPTIDES CALPAIN |
| ISSN号 | 0340-1022 |
| 通讯作者 | przybylski m |
| 中文摘要 | the combination of maldi-tof-mass spectrometry with gel electrophoretic separation using protein visualization by staining procedures involving such as coomassie brilliant blue has been established as a widely used approach in proteomics. although this approach has been shown to present high detection sensitivity, drawbacks and limitations frequently arise from the significant background in the mass spectrometric analysis. in this chapter we describe an approach for the application of maldi-ms to the mass spectrometric identification of proteins from one-dimensional (1d) and two-dimensional (2d) gel electrophoretic separation, using stain-free detection and visualization based on native protein fluorescence. using the native fluorescence of aromatic protein amino acids with uv transmission at 343 nm as a fast gel imaging system, unstained protein spots are localized and, upon excision from gels, can be proteolytically digested and analyzed by maldi-ms. following the initial development and testing with standard proteins, applications of the stain-free gel electrophoretic detection approach to mass spectrometric identification of biological proteins from 2d-gel separations clearly show the feasibility and efficiency of this combination, as illustrated by a proteomics study of porcine skeleton muscle proteins. major advantages of the stain-free gel detection approach with maldi-ms analysis are (1) rapid analysis of proteins from 1d- and 2d-gel separation without destaining required prior to proteolytic digestion, (2) the low detection limits of proteins attained, and (3) low background in the maldi-ms analysis. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321616300003 |
| 公开日期 | 2014-04-18 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50245]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Susnea I,Bernevic B,Wicke M,et al. Application of MALDI-TOF-Mass Spectrometry to Proteome Analysis Using Stain-Free Gel Electrophoresis[J]. applications of maldi-tof spectroscopy,2013,331:37-54. |
| APA | Susnea I.,Bernevic B.,Wicke M.,Ma L.,Liu SY.,...&Przybylski M.(2013).Application of MALDI-TOF-Mass Spectrometry to Proteome Analysis Using Stain-Free Gel Electrophoresis.applications of maldi-tof spectroscopy,331,37-54. |
| MLA | Susnea I,et al."Application of MALDI-TOF-Mass Spectrometry to Proteome Analysis Using Stain-Free Gel Electrophoresis".applications of maldi-tof spectroscopy 331(2013):37-54. |
入库方式： OAI收割
来源：长春应用化学研究所
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