Determination of Peptide and Protein Disulfide Linkages by MALDI Mass Spectrometry
文献类型：期刊论文
| 作者 | Yang HM ; Liu N ; Liu SY |
| 刊名 | applications of maldi-tof spectroscopy
 |
| 出版日期 | 2013 |
| 卷号 | 331页码:79-116 |
| 关键词 | ASSISTED LASER-DESORPTION ELECTRON-CAPTURE DISSOCIATION TIME-OF-FLIGHT PHOTOACTIVE YELLOW PROTEIN IN-SOURCE DECAY P-COUMARIC ACID CYSTEINE-CONTAINING PEPTIDES NEGATIVE SIGNATURE MASS CYSTINYL PROTEINS IONIZATION MALDI |
| ISSN号 | 0340-1022 |
| 通讯作者 | liu sy |
| 中文摘要 | the disulfide bond is one of the most common post-translational modifications in proteins, of which determination is essential to the comprehensive understanding of protein structures. disulfide bond analysis has undergone great improvement due to the development of matrix-assisted laser desorption/ionization mass spectrometry (maldi ms), especially in terms of speed and sensitivity. in general, the characterization of disulfide-containing peptides is achieved by the reduction of disulfide bonds followed by alkylation. in this review we focus on the analysis of disulfide-containing proteins/peptides by some unique methods in maldi ms. the maldi in-source decay (isd) of disulfide bonds and adducts of the matrix and sulfhydryl-containing peptide are discussed in detail. the mechanism of each method is discussed so as to help the reader gain greater insight into it, and examples of its application are also presented. the goal of this review is to provide an understanding of these techniques for analysis of disulfide-linked proteins/peptides in maldi ms. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321616300005 |
| 公开日期 | 2014-04-18 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50246]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang HM,Liu N,Liu SY. Determination of Peptide and Protein Disulfide Linkages by MALDI Mass Spectrometry[J]. applications of maldi-tof spectroscopy,2013,331:79-116. |
| APA | Yang HM,Liu N,&Liu SY.(2013).Determination of Peptide and Protein Disulfide Linkages by MALDI Mass Spectrometry.applications of maldi-tof spectroscopy,331,79-116. |
| MLA | Yang HM,et al."Determination of Peptide and Protein Disulfide Linkages by MALDI Mass Spectrometry".applications of maldi-tof spectroscopy 331(2013):79-116. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。