Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell
文献类型：期刊论文
| 作者 | Li YJ ; Qiao HY ; Yan W ; Zhang J ; Xing CY ; Wang HD ; Zhang BL ; Tang JL |
| 刊名 | journal of molecular recognition
 |
| 出版日期 | 2013 |
| 卷号 | 26期号:1页码:46-50 |
| 关键词 | CARBOHYDRATE INTERACTION EXPONENTIAL ENRICHMENT SYSTEMATIC EVOLUTION BINDING APTAMER NUCLEIC-ACID X-RAY MICROSCOPY LIGANDS DNA AFM |
| ISSN号 | 0952-3499 |
| 通讯作者 | zhang bl |
| 中文摘要 | molecular recognition force spectroscopy (mr-fs) was applied to investigate the dynamic interaction between aptamer gbi-10 and tenascin-c (tn-c) on human glioblastoma cell surface at single-molecule level. the unbinding force between aptamer gbi-10 and tn-c was 39?pn at the loading rate of 0.3?nn?sec-1. a series of kinetic parameters concerning interaction process such as the unbinding force fu, the association rate constant kon, dissociation rate constant at zero force koff, and dissociation constant kd for aptamer gbi-10/tn-c complexes were acquired. in addition, the interaction of aptamer gbi-10 with tn-c depended on the presence of mg2+. this work demonstrates that mr-fs can be used as an attractive tool for exploring the interaction forces and dynamic process of aptamer and ligand at the single-molecule level. as a future perspective, mr-fs may be used as a potential diagnostic and therapeutic tool by combining with other techniques. copyright (c) 2012 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312886800007 |
| 公开日期 | 2014-04-18 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50420]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li YJ,Qiao HY,Yan W,et al. Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell[J]. journal of molecular recognition,2013,26(1):46-50. |
| APA | Li YJ.,Qiao HY.,Yan W.,Zhang J.,Xing CY.,...&Tang JL.(2013).Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell.journal of molecular recognition,26(1),46-50. |
| MLA | Li YJ,et al."Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell".journal of molecular recognition 26.1(2013):46-50. |
入库方式： OAI收割
来源：长春应用化学研究所
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