Fabricating three-dimensional hydrogel oligonucleotide microarrays to detect single nucleotide polymorphisms
文献类型：期刊论文
| 作者 | Liu X ; Li T ; Liu DJ ; Wang ZX |
| 刊名 | analytical methods
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:1页码:285-290 |
| 关键词 | DNA MICROARRAYS BIOSENSOR APPLICATIONS PROTEIN MICROCHIPS MICROFLUIDIC CHANNELS ENZYMATIC-REACTIONS MODIFIED SURFACE CHAIN-REACTION NUCLEIC-ACIDS GLASS SLIDES ARRAYS |
| ISSN号 | 1759-9660 |
| 通讯作者 | liu dj |
| 中文摘要 | here, a three-dimensional (3d) oligonucleotide microarray-based fluorescent assay has been developed for single nucleotide polymorphisms (snps) and allele frequency determination. the 3d oligonucleotide microarray is fabricated on a home-made polyacrylamide hydrogel microarray which is generated by uv light photopolymerization of tiny acrylamide monomer droplets on a peg terminated glass slide. after activation by glutaraldehyde (ga), the polyacrylamide hydrogel is able to react with nh2-modified oligonucleotides with high efficiency to generate a high signal-to-background ratio without the conventional blocking step. we demonstrate that the assay is able to detect complimentary 25 mer oligonucleotide target (fragment of a tumor suppressor gene p53) down to 100 pm and snp with true-to-false ratios (the ratio of fluorescence signal from perfect matched dna strands to that from the one-base mismatched dna strands) above 20. taking advantage of the high specificity of the assay, allele frequency as low as 2% can be accurately determined. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000311968000036 |
| 公开日期 | 2014-04-18 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50444]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu X,Li T,Liu DJ,et al. Fabricating three-dimensional hydrogel oligonucleotide microarrays to detect single nucleotide polymorphisms[J]. analytical methods,2013,5(1):285-290. |
| APA | Liu X,Li T,Liu DJ,&Wang ZX.(2013).Fabricating three-dimensional hydrogel oligonucleotide microarrays to detect single nucleotide polymorphisms.analytical methods,5(1),285-290. |
| MLA | Liu X,et al."Fabricating three-dimensional hydrogel oligonucleotide microarrays to detect single nucleotide polymorphisms".analytical methods 5.1(2013):285-290. |
入库方式： OAI收割
来源：长春应用化学研究所
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